General fitness, health and nutrition · Public discussion

vitamin E levels acts as a protective factor

Doe · 2004-02-15T20:47:24+00:00

Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links Effect of an acute dose of ethanol on lipid peroxidation in rats: action of vitamin E. Jordao AA Jr, Chiarello PG, Arantes MR, Meirelles MS, Vannucchi H. Postgraduation in Food Science, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirao Preto-SP, Brazil. Free radical generation is an important step in the pathogenesis of ethanol-associated liver injury. Administration of ethanol induces an increase in lipid peroxidation both by enhancing the production of oxygen reactive species and by decreasing the levels of endogenous antioxidants. This work focuses on the generation of free radicals provoked by an acute ethanol dose in rats, and the role of different dietary levels of vitamin E. The objective of this investigation was to study the effect of three different dietary levels of vitamin E (deficient, control and supplemented with 20 times higher levels) on plasma and liver lipid peroxidation (assayed by TBARS), vitamin E in plasma and liver, and hepatic glutathione concentration, in rats receiving the different diets. The animals were submitted to an acute dose of ethanol (5 g/kg body weight) administered by gavage at the end of an experimental 4 week period and were sacrificed at 0, 2, 4, 8 and 24 h after ethanol administration. Dietary vitamin E caused a dose-dependent increase in liver and plasma concentration of the vitamin, but ethanol administration decreased hepatic vitamin E in all groups. TBARS concentrations were higher in liver of rats that received the deficient diet, independent of ethanol, however, liver TBARS concentrations were low in control and supplemented groups, but increased with ethanol ingestion. Glutathione levels were lowered by ethanol administration in all groups, in different times, but recovered to this original level in 24 h time. In conclusion, vitamin E deficiency alone induces liver lipid peroxidation in rats, acute administration of ethanol affect vitamin E and GSH level and maintenance of adequate or higher vitamin E levels acts as a protective factor against free radical generation. PMID: 14871588 [PubMed - in process] -------------------------------------------------------------------------- ------ Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://pages.ivillage.com/ironjustice/manisaherbivore DEAD PEOPLE WALKING http://pages.ivillage.com/ironjustice/deadpeoplewalking

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01 / Original postvitamin E levels acts as a protective factorDoe · 15 Feb 2004 02 / This pagePosts 1–13Page 1 of 1 · chronological order 03 / Latest replyPost #13Manky Badger · 22 Feb 2004

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Original section: General fitness, health and nutrition
Published: 15 February 2004
Last activity: 22 February 2004
Original author: Doe
Posts: 13
Discussion status: Public discussion
Total views: 972
Views / 30 days: 0

The navigation and discussion metadata provide context. Posts remain in their original chronological order.

Original post
Doe
Archived post

15 February 2004, 20:47 UTC #1

Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links
Effect of an acute dose of ethanol on lipid peroxidation in rats: action of vitamin E.
Jordao AA Jr, Chiarello PG, Arantes MR, Meirelles MS, Vannucchi H.
Postgraduation in Food Science, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Av.
Bandeirantes, 3900, 14049-900, Ribeirao Preto-SP, Brazil.
Free radical generation is an important step in the pathogenesis of ethanol-associated liver injury.
Administration of ethanol induces an increase in lipid peroxidation both by enhancing the production
of oxygen reactive species and by decreasing the levels of endogenous antioxidants. This work
focuses on the generation of free radicals provoked by an acute ethanol dose in rats, and the role
of different dietary levels of vitamin E. The objective of this investigation was to study the
effect of three different dietary levels of vitamin E (deficient, control and supplemented with 20
times higher levels) on plasma and liver lipid peroxidation (assayed by TBARS), vitamin E in plasma
and liver, and hepatic glutathione concentration, in rats receiving the different diets. The animals
were submitted to an acute dose of ethanol (5 g/kg body weight) administered by gavage at the end of
an experimental 4 week period and were sacrificed at 0, 2, 4, 8 and 24 h after ethanol
administration. Dietary vitamin E caused a dose-dependent increase in liver and plasma concentration
of the vitamin, but ethanol administration decreased hepatic vitamin E in all groups. TBARS
concentrations were higher in liver of rats that received the deficient diet, independent of
ethanol, however, liver TBARS concentrations were low in control and supplemented groups, but
increased with ethanol ingestion. Glutathione levels were lowered by ethanol administration in all
groups, in different times, but recovered to this original level in 24 h time. In conclusion,
vitamin E deficiency alone induces liver lipid peroxidation in rats, acute administration of ethanol
affect vitamin E and GSH level and maintenance of adequate or higher vitamin E levels acts as a
protective factor against free radical generation.
PMID: 14871588 [PubMed - in process]
--------------------------------------------------------------------------
------
Who loves ya. Tom Jesus Was A Vegetarian! jesuswasavegetarian.7h.comjesuswasavegetarian.7h.com Man Is A Herbivore!
pages.ivillage.commanisaherbivore DEAD PEOPLE WALKING
pages.ivillage.comdeadpeoplewalking

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Manky Badger
Archived post

15 February 2004, 22:17 UTC #2

"doe" <[email hidden]> wrote in message
"]news:[email hidden]...
Quoted message said:
Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links
Effect of an acute dose of ethanol on lipid peroxidation in rats: action

of
Quoted message said:
vitamin E.
Speaking as one who's actually looked into this recently.........
The article I've snipped doesn't go into specific details about the technique by which vitamin E was
measured. There are a few methods available, most of which are rather time consuming, and many of
which aren't very precise. Without more details about the reproducubility of the assay employed, I'd
be rather sceptical about the article.
MB

Report
markd
Archived post

15 February 2004, 22:49 UTC #3

What are we to make of this, to what practical application, to what broader body of knowledge, to
what theoretical approach to what set of questions? At least rats who fall off the wagon know vit e
might be of some use for their livers.
Quoted message said:
Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links
Effect of an acute dose of ethanol on lipid peroxidation in rats: action of vitamin E.
Jordao AA Jr, Chiarello PG, Arantes MR, Meirelles MS, Vannucchi H.
Postgraduation in Food Science, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Av.
Bandeirantes, 3900, 14049-900, Ribeirao Preto-SP, Brazil.
Free radical generation is an important step in the pathogenesis of ethanol-associated liver
injury. Administration of ethanol induces an increase in lipid peroxidation both by enhancing the
production of oxygen reactive species and by decreasing the levels of endogenous antioxidants. This
work focuses on the generation of free radicals provoked by an acute ethanol dose in rats, and the
role of different dietary levels of vitamin E. The objective of this investigation was to study the
effect of three different dietary levels of vitamin E (deficient, control and supplemented with 20
times higher levels) on plasma and liver lipid peroxidation (assayed by TBARS), vitamin E in plasma
and liver, and hepatic glutathione concentration, in rats receiving the different diets. The
animals were submitted to an acute dose of ethanol (5 g/kg body weight) administered by gavage at
the end of an experimental 4 week period and were sacrificed at 0, 2, 4, 8 and 24 h after ethanol
administration. Dietary vitamin E caused a dose-dependent increase in liver and plasma
concentration of the vitamin, but ethanol administration decreased hepatic vitamin E in all groups.
TBARS concentrations were higher in liver of rats that received the deficient diet, independent of
ethanol, however, liver TBARS concentrations were low in control and supplemented groups, but
increased with ethanol ingestion. Glutathione levels were lowered by ethanol administration in all
groups, in different times, but recovered to this original level in 24 h time. In conclusion,
vitamin E deficiency alone induces liver lipid peroxidation in rats, acute administration of
ethanol affect vitamin E and GSH level and maintenance of adequate or higher vitamin E levels acts
as a protective factor against free radical generation.
PMID: 14871588 [PubMed - in process]
--------------------------------------------------------------------------
------
Who loves ya. Tom Jesus Was A Vegetarian! jesuswasavegetarian.7h.comjesuswasavegetarian.7h.com Man Is A Herbivore!
pages.ivillage.commanisaherbivore DEAD PEOPLE WALKING
pages.ivillage.comdeadpeoplewalking

Report
Jeffrey Turner
Archived post

16 February 2004, 03:30 UTC #4

Quoted message said:
What are we to make of this, to what practical application, to what broader body of knowledge, to
what theoretical approach to what set of questions? At least rats who fall off the wagon know vit
e might be of some use for their livers.
Vitamin E enriched beer?
Quoted message said:
Quoted message said:
Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links
Effect of an acute dose of ethanol on lipid peroxidation in rats: action of vitamin E.
Jordao AA Jr, Chiarello PG, Arantes MR, Meirelles MS, Vannucchi H.
Postgraduation in Food Science, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Av.
Bandeirantes, 3900, 14049-900, Ribeirao Preto-SP, Brazil.
Free radical generation is an important step in the pathogenesis of ethanol-associated liver
injury. Administration of ethanol induces an increase in lipid peroxidation both by enhancing the
production of oxygen reactive species and by decreasing the levels of endogenous antioxidants.
This work focuses on the generation of free radicals provoked by an acute ethanol dose in rats,
and the role of different dietary levels of vitamin E. The objective of this investigation was to
study the effect of three different dietary levels of vitamin E (deficient, control and
supplemented with 20 times higher levels) on plasma and liver lipid peroxidation (assayed by
TBARS), vitamin E in plasma and liver, and hepatic glutathione concentration, in rats receiving
the different diets. The animals were submitted to an acute dose of ethanol (5 g/kg body weight)
administered by gavage at the end of an experimental 4 week period and were sacrificed at 0, 2, 4,
8 and 24 h after ethanol administration. Dietary vitamin E caused a dose-dependent increase in
liver and plasma concentration of the vitamin, but ethanol administration decreased hepatic
vitamin E in all groups. TBARS concentrations were higher in liver of rats that received the
deficient diet, independent of ethanol, however, liver TBARS concentrations were low in control
and supplemented groups, but increased with ethanol ingestion. Glutathione levels were lowered by
ethanol administration in all groups, in different times, but recovered to this original level in
24 h time. In conclusion, vitamin E deficiency alone induces liver lipid peroxidation in rats,
acute administration of ethanol affect vitamin E and GSH level and maintenance of adequate or
higher vitamin E levels acts as a protective factor against free radical generation.
PMID: 14871588 [PubMed - in process]
--------------------------------------------------------------------------
------
Who loves ya. Tom Jesus Was A Vegetarian! jesuswasavegetarian.7h.comjesuswasavegetarian.7h.com Man Is A Herbivore!
pages.ivillage.commanisaherbivore DEAD PEOPLE WALKING
pages.ivillage.comdeadpeoplewalking

--
Ho, ho, ho, hee, hee, hee and a couple of ha, ha, has; That's how we pass the day away, in the merry
old land of Oz.

Report
Doe
Archived post

17 February 2004, 01:24 UTC #5

Quoted message said:
Subject: Re: vitamin E levels acts as a protective factor
From: [email hidden]
Date: 2/15/2004 2:31 PM Mountain Standard Time
Message-id: <[email hidden]>
What are we to make of this, to what practical application, to what broader body of knowledge, to
what theoretical approach to what set of questions? At least rats who fall off the wagon know vit e
might be of some use for their livers.
Arch Biochem Biophys. 2004 Mar 1;423(1):162-9. Related Articles, Links
Enhanced inflammatory responses in alpha-tocopherol transfer protein null mice.
Schock BC, Van Der Vliet A, Corbacho AM, Leonard SW, Finkelstein E, Valacchi G, Obermueller-Jevic U,
Cross CE, Traber MG.
Division of Pulmonary and Critical Care Medicine and Center for Comparative Respiratory Biology and
Medicine, University of California School of Medicine, Davis, CA 95616, USA.
The liver preferentially secretes alpha-tocopherol into plasma under the control of the hepatic alpha-
tocopherol transfer protein (alpha-TTP). alpha-TTP-null mice (Ttpa(-/-) mice) are vitamin E
deficient, therefore were used for investigations of in vivo responses to sub-normal tissue alpha-
tocopherol concentrations during inflammation. Increased basal oxidative stress in Ttpa(-/-) mice
was documented by increased plasma lipid peroxidation, and superoxide production by bone marrow-
derived neutrophils stimulated in vitro with phorbol 12-myristate 13-acetate. Lipopolysaccharide
(LPS) injected intraperitoneally induced increases in lung and liver HO-1 and iNOS, as well as
plasma NO(x) in Ttpa(+/+) mice. LPS induced more modest increases in these markers in Ttpa(-/-)
mice, while more marked increases in plasma IL-10 and lung lavage TNFalpha were observed. Taken
together, these results demonstrate that alpha-tocopherol is important for proper modulation of
inflammatory responses and that sub-optimal alpha-tocopherol concentrations may derange inflammatory-
immune responses.
PMID: 14871478 [PubMed - in process]
--------------------------------------------------------------------------
------
Quoted message said:
Quoted message said:
Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links
Effect of an acute dose of ethanol on lipid peroxidation in rats: action of vitamin E.
Jordao AA Jr, Chiarello PG, Arantes MR, Meirelles MS, Vannucchi H.
Postgraduation in Food Science, Faculty of Pharmaceutical Sciences,

University
Quoted message said:
of Sao Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirao Preto-SP, Brazil.
Free radical generation is an important step in the pathogenesis of ethanol-associated liver
injury. Administration of ethanol induces an

increase
Quoted message said:
in lipid peroxidation both by enhancing the production of oxygen reactive species and by
decreasing the levels of endogenous antioxidants. This work focuses on the generation of free
radicals provoked by an acute ethanol dose

in
Quoted message said:
rats, and the role of different dietary levels of vitamin E. The objective

of
Quoted message said:
this investigation was to study the effect of three different dietary levels

of
Quoted message said:
vitamin E (deficient, control and supplemented with 20 times higher levels)

on
Quoted message said:
plasma and liver lipid peroxidation (assayed by TBARS), vitamin E in plasma

and
Quoted message said:
liver, and hepatic glutathione concentration, in rats receiving the

different
Quoted message said:
diets. The animals were submitted to an acute dose of ethanol (5 g/kg body weight) administered by
gavage at the end of an experimental 4 week period

and
Quoted message said:
were sacrificed at 0, 2, 4, 8 and 24 h after ethanol administration. Dietary vitamin E caused a
dose-dependent increase in liver and plasma concentration

of
Quoted message said:
the vitamin, but ethanol administration decreased hepatic vitamin E in all groups. TBARS
concentrations were higher in liver of rats that received the deficient diet, independent of
ethanol, however, liver TBARS concentrations were low in control and supplemented groups, but
increased with ethanol ingestion. Glutathione levels were lowered by ethanol administration in all
groups, in different times, but recovered to this original level in 24 h

time.
Quoted message said:
In conclusion, vitamin E deficiency alone induces liver lipid peroxidation

in
Quoted message said:
rats, acute administration of ethanol affect vitamin E and GSH level and maintenance of adequate
or higher vitamin E levels acts as a protective

factor
Quoted message said:
against free radical generation.
PMID: 14871588 [PubMed - in process]
--------------------------------------------------------------------------
------
Who loves ya. Tom
Jesus Was A Vegetarian! jesuswasavegetarian.7h.comjesuswasavegetarian.7h.com Man Is A Herbivore!
pages.ivillage.commanisaherbivore DEAD PEOPLE WALKING
pages.ivillage.comdeadpeoplewalking

Report
Doe
Archived post

17 February 2004, 01:24 UTC #6

Quoted message said:
Subject: Re: vitamin E levels acts as a protective factor
From: "Manky Badger" [email hidden]
Date: 2/15/2004 2:09 PM Mountain Standard Time
Message-id: <[email hidden]>
"doe" <[email hidden]> wrote in message news:20040215150512.13026.00002233@mb-
m02.aol.com...
Quoted message said:
Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links
Effect of an acute dose of ethanol on lipid peroxidation in rats: action

of
Quoted message said:
vitamin E.
Speaking as one who's actually looked into this recently.........
The article I've snipped doesn't go into specific details about the technique by which vitamin E
was measured. There are a few methods available, most of which are rather time consuming, and many
of which aren't very precise. Without more details about the reproducubility of the assay employed,
I'd be rather sceptical about the article.
MB
Arch Biochem Biophys. 2004 Mar 1;423(1):162-9. Related Articles, Links
Enhanced inflammatory responses in alpha-tocopherol transfer protein null mice.
Schock BC, Van Der Vliet A, Corbacho AM, Leonard SW, Finkelstein E, Valacchi G, Obermueller-Jevic U,
Cross CE, Traber MG.
Division of Pulmonary and Critical Care Medicine and Center for Comparative Respiratory Biology and
Medicine, University of California School of Medicine, Davis, CA 95616, USA.
The liver preferentially secretes alpha-tocopherol into plasma under the control of the hepatic alpha-
tocopherol transfer protein (alpha-TTP). alpha-TTP-null mice (Ttpa(-/-) mice) are vitamin E
deficient, therefore were used for investigations of in vivo responses to sub-normal tissue alpha-
tocopherol concentrations during inflammation. Increased basal oxidative stress in Ttpa(-/-) mice
was documented by increased plasma lipid peroxidation, and superoxide production by bone marrow-
derived neutrophils stimulated in vitro with phorbol 12-myristate 13-acetate. Lipopolysaccharide
(LPS) injected intraperitoneally induced increases in lung and liver HO-1 and iNOS, as well as
plasma NO(x) in Ttpa(+/+) mice. LPS induced more modest increases in these markers in Ttpa(-/-)
mice, while more marked increases in plasma IL-10 and lung lavage TNFalpha were observed. Taken
together, these results demonstrate that alpha-tocopherol is important for proper modulation of
inflammatory responses and that sub-optimal alpha-tocopherol concentrations may derange inflammatory-
immune responses.
PMID: 14871478 [PubMed - in process]
--------------------------------------------------------------------------
------
Who loves ya. Tom Jesus Was A Vegetarian! jesuswasavegetarian.7h.comjesuswasavegetarian.7h.com Man Is A Herbivore!
pages.ivillage.commanisaherbivore DEAD PEOPLE WALKING
pages.ivillage.comdeadpeoplewalking

Report
Doe
Archived post

17 February 2004, 01:24 UTC #7

Quoted message said:
Subject: Re: vitamin E levels acts as a protective factor
From: [email hidden]
Date: 2/15/2004 2:31 PM Mountain Standard Time
Message-id: <[email hidden]>
What are we to make of this, to what practical application, to what broader body of knowledge, to
what theoretical approach to what set of questions? At least rats who fall off the wagon know vit e
might be of some use for their livers.
Arch Biochem Biophys. 2004 Mar 1;423(1):162-9. Related Articles, Links
Enhanced inflammatory responses in alpha-tocopherol transfer protein null mice.
Schock BC, Van Der Vliet A, Corbacho AM, Leonard SW, Finkelstein E, Valacchi G, Obermueller-Jevic U,
Cross CE, Traber MG.
Division of Pulmonary and Critical Care Medicine and Center for Comparative Respiratory Biology and
Medicine, University of California School of Medicine, Davis, CA 95616, USA.
The liver preferentially secretes alpha-tocopherol into plasma under the control of the hepatic alpha-
tocopherol transfer protein (alpha-TTP). alpha-TTP-null mice (Ttpa(-/-) mice) are vitamin E
deficient, therefore were used for investigations of in vivo responses to sub-normal tissue alpha-
tocopherol concentrations during inflammation. Increased basal oxidative stress in Ttpa(-/-) mice
was documented by increased plasma lipid peroxidation, and superoxide production by bone marrow-
derived neutrophils stimulated in vitro with phorbol 12-myristate 13-acetate. Lipopolysaccharide
(LPS) injected intraperitoneally induced increases in lung and liver HO-1 and iNOS, as well as
plasma NO(x) in Ttpa(+/+) mice. LPS induced more modest increases in these markers in Ttpa(-/-)
mice, while more marked increases in plasma IL-10 and lung lavage TNFalpha were observed. Taken
together, these results demonstrate that alpha-tocopherol is important for proper modulation of
inflammatory responses and that sub-optimal alpha-tocopherol concentrations may derange inflammatory-
immune responses.
PMID: 14871478 [PubMed - in process]
--------------------------------------------------------------------------
------
Quoted message said:
Quoted message said:
Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links
Effect of an acute dose of ethanol on lipid peroxidation in rats: action of vitamin E.
Jordao AA Jr, Chiarello PG, Arantes MR, Meirelles MS, Vannucchi H.
Postgraduation in Food Science, Faculty of Pharmaceutical Sciences,

University
Quoted message said:
of Sao Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirao Preto-SP, Brazil.
Free radical generation is an important step in the pathogenesis of ethanol-associated liver
injury. Administration of ethanol induces an

increase
Quoted message said:
in lipid peroxidation both by enhancing the production of oxygen reactive species and by
decreasing the levels of endogenous antioxidants. This work focuses on the generation of free
radicals provoked by an acute ethanol dose

in
Quoted message said:
rats, and the role of different dietary levels of vitamin E. The objective

of
Quoted message said:
this investigation was to study the effect of three different dietary levels

of
Quoted message said:
vitamin E (deficient, control and supplemented with 20 times higher levels)

on
Quoted message said:
plasma and liver lipid peroxidation (assayed by TBARS), vitamin E in plasma

and
Quoted message said:
liver, and hepatic glutathione concentration, in rats receiving the

different
Quoted message said:
diets. The animals were submitted to an acute dose of ethanol (5 g/kg body weight) administered by
gavage at the end of an experimental 4 week period

and
Quoted message said:
were sacrificed at 0, 2, 4, 8 and 24 h after ethanol administration. Dietary vitamin E caused a
dose-dependent increase in liver and plasma concentration

of
Quoted message said:
the vitamin, but ethanol administration decreased hepatic vitamin E in all groups. TBARS
concentrations were higher in liver of rats that received the deficient diet, independent of
ethanol, however, liver TBARS concentrations were low in control and supplemented groups, but
increased with ethanol ingestion. Glutathione levels were lowered by ethanol administration in all
groups, in different times, but recovered to this original level in 24 h

time.
Quoted message said:
In conclusion, vitamin E deficiency alone induces liver lipid peroxidation

in
Quoted message said:
rats, acute administration of ethanol affect vitamin E and GSH level and maintenance of adequate
or higher vitamin E levels acts as a protective

factor
Quoted message said:
against free radical generation.
PMID: 14871588 [PubMed - in process]
--------------------------------------------------------------------------
------
Who loves ya. Tom
Jesus Was A Vegetarian! jesuswasavegetarian.7h.comjesuswasavegetarian.7h.com Man Is A Herbivore!
pages.ivillage.commanisaherbivore DEAD PEOPLE WALKING
pages.ivillage.comdeadpeoplewalking

Report
Manky Badger
Archived post

17 February 2004, 15:17 UTC #8

"doe" <[email hidden]> wrote in message
"]news:[email hidden]...
Quoted message said:
Quoted message said:
Subject: Re: vitamin E levels acts as a protective factor From: "Manky Badger"
[email hidden] Date: 2/15/2004 2:09 PM Mountain Standard Time Message-
id: <[email hidden]>
"doe" <[email hidden]> wrote in message news:20040215150512.13026.00002233@mb-
m02.aol.com...
Quoted message said:
Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links
Effect of an acute dose of ethanol on lipid peroxidation in rats:

action
Quoted message said:
Quoted message said:
of
Quoted message said:
vitamin E.
Speaking as one who's actually looked into this recently.........
The article I've snipped doesn't go into specific details about the technique by which vitamin E
was measured. There are a few methods available, most of which are rather time

consuming,
Quoted message said:
Quoted message said:
and many of which aren't very precise. Without more details about the reproducubility of the
assay employed, I'd

be
Quoted message said:
Quoted message said:
rather sceptical about the article.
MB
Arch Biochem Biophys. 2004 Mar 1;423(1):162-9. Related Articles, Links
Enhanced inflammatory responses in alpha-tocopherol transfer protein null

mice.
Quoted message said:

Schock BC, Van Der Vliet A, Corbacho AM, Leonard SW, Finkelstein E,

Valacchi G,
Quoted message said:
Obermueller-Jevic U, Cross CE, Traber MG.
Division of Pulmonary and Critical Care Medicine and Center for

Comparative
Quoted message said:
Respiratory Biology and Medicine, University of California School of

Medicine,
Quoted message said:
Davis, CA 95616, USA.
The liver preferentially secretes alpha-tocopherol into plasma under the control of the hepatic
alpha-tocopherol transfer protein (alpha-TTP). alpha-TTP-null mice (Ttpa(-/-) mice) are vitamin E
deficient, therefore

were
Quoted message said:
used for investigations of in vivo responses to sub-normal tissue alpha-tocopherol concentrations
during inflammation. Increased basal

oxidative
Quoted message said:
stress in Ttpa(-/-) mice was documented by increased plasma lipid

peroxidation,
Quoted message said:
and superoxide production by bone marrow-derived neutrophils stimulated in vitro with phorbol 12-
myristate 13-acetate. Lipopolysaccharide (LPS)

injected
Quoted message said:
intraperitoneally induced increases in lung and liver HO-1 and iNOS, as

well as
Quoted message said:
plasma NO(x) in Ttpa(+/+) mice. LPS induced more modest increases in these markers in Ttpa(-/-)
mice, while more marked increases in plasma IL-10 and

lung
Quoted message said:
lavage TNFalpha were observed. Taken together, these results demonstrate

that
Quoted message said:
alpha-tocopherol is important for proper modulation of inflammatory

responses
Quoted message said:
and that sub-optimal alpha-tocopherol concentrations may derange inflammatory-immune responses.
Yes, all very well, but still doesn't give anything about how plausible the actuall assay is. I'm
assured by "those who really know" that some vitamin E assays give a valid result, and others are
effectively random number generators. Without knowing the specifics of the assay used in this study,
the findings must remain subject to question.
MB

Report
Doe
Archived post

19 February 2004, 21:13 UTC #9

Quoted message said:
Subject: Re: vitamin E levels acts as a protective factor
From: "Manky Badger" [email hidden]
Date: 2/15/2004 2:09 PM Mountain Standard Time
Message-id: <[email hidden]>
"doe" <[email hidden]> wrote in message news:20040215150512.13026.00002233@mb-
m02.aol.com...
Quoted message said:
Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links
Effect of an acute dose of ethanol on lipid peroxidation in rats: action

of
Quoted message said:
vitamin E.
Speaking as one who's actually looked into this recently.........
The article I've snipped doesn't go into specific details about the technique by which vitamin E
was measured. There are a few methods available, most of which are rather time consuming, and many
of which aren't very precise. Without more details about the reproducubility of the assay employed,
I'd be rather sceptical about the article.
MB
These guys seem to have agreed upon a method of testing .. ?
Wiad Lek. 2003;56(7-8):337-40. Related Articles, Links
Antioxidant status in newborns and infants suffering from congenital heart defects.
Rokicki W, Strzalkowski A, Klapcinska B, Danch A, Sobczak A.
Department of Pediatric Cardiology Medical University of Silesia, Katowice.
There is a common view that free radicals may play an important role in tissue damage resulting from
circulatory insufficiency, cardiosurgery etc. There are very few data concerning the involvement of
free radical reactions in the newborns and infants suffering from congenital heart defects (CHD).
Antioxidant status was evaluated in 41 newborns and infants under 1 year of age, among them 23
suffering from CHD (14 with left-to-right shunt and 9 with cyanotic heart defect) and 18 healthy
controls. The study based on the assessment of activities of antioxidant enzymes in blood
(superoxide dismutase, catalase and glutathione peroxidase), levels of low molecular weight
antioxidants (vitamin E, uric acid and selenium) and the concentration of malondialdehyde (MDA) as a
marker of lipid peroxidation. All subjects had low blood selenium concentration as compared to the
level considered as being adequate. Infants suffering from CHD had lower, as compared to healthy
controls, plasma vitamin E concentration. The difference was significant in the case of acyanotic
ones. The activities of superoxide dismutase and catalase in infants with CHD were not significantly
different from the respective values recorded in healthy controls. The activity of glutathione
peroxidase in whole blood was the lowest in infants with cyanotic heart defect in whom lipid
peroxidation, as evaluated by MDA level, was the most enhanced. Significantly higher plasma
concentration of uric acid which may be interpreted as a positive mechanism enabling better
protection of red blood cells from peroxidative damage was found in this group of infants. It is
concluded that enhanced oxidative stress due to imbalance between prooxidant and antioxidant
reactions appears to be associated with congenital heart defect pathology in infants.
PMID: 14969161 [PubMed - in process]
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Manky Badger
Archived post

19 February 2004, 21:13 UTC #10

"doe" <[email hidden]> wrote in message
"]news:[email hidden]...
Quoted message said:
Quoted message said:
Subject: Re: vitamin E levels acts as a protective factor From: "Manky Badger"
[email hidden] Date: 2/15/2004 2:09 PM Mountain Standard Time Message-
id: <[email hidden]>
"doe" <[email hidden]> wrote in message news:20040215150512.13026.00002233@mb-
m02.aol.com...
Quoted message said:
Food Chem Toxicol. 2004 Mar;42(3):459-64. Related Articles, Links
Effect of an acute dose of ethanol on lipid peroxidation in rats:

action
Quoted message said:
Quoted message said:
of
Quoted message said:
vitamin E.
Speaking as one who's actually looked into this recently.........
The article I've snipped doesn't go into specific details about the technique by which vitamin E
was measured. There are a few methods available, most of which are rather time

consuming,
Quoted message said:
Quoted message said:
and many of which aren't very precise. Without more details about the reproducubility of the
assay employed, I'd

be
Quoted message said:
Quoted message said:
rather sceptical about the article.
MB
These guys seem to have agreed upon a method of testing .. ?
Wiad Lek. 2003;56(7-8):337-40. Related Articles, Links
Antioxidant status in newborns and infants suffering from congenital heart defects.
Rokicki W, Strzalkowski A, Klapcinska B, Danch A, Sobczak A.
Department of Pediatric Cardiology Medical University of Silesia,

Katowice.
snip
They may well have but the article gives no details at all about the technique used to measure
vitamin E.
MB

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Manky Badger
Archived post

21 February 2004, 17:48 UTC #11

"doe" <[email hidden]> wrote in message
"]news:[email hidden]...
Quoted message said:
Quoted message said:
They may well have but the article gives no details at all about the technique used to measure
vitamin E.
MB
Well .. do some research ..
Maybe you will find out what they used ..
Maybe you will be able to ALSO use the same method ..
Then again .. maybe .. not ..
Half full or half empty ..
You've got the article Tom - you tell me.
MB
"Cytoadhesion" - LOL !!!

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Manky Badger
Archived post

22 February 2004, 00:19 UTC #12

"doe" <[email hidden]> wrote in message
"]news:[email hidden]...
Quoted message said:
Quoted message said:
Subject: Re: vitamin E levels acts as a protective factor From: "Manky Badger"
[email hidden] Date: 2/21/2004 9:39 AM Mountain Standard Time Message-
id: <[email hidden]>
"doe" <[email hidden]> wrote in message news:20040219232452.16260.00000020@mb-
m11.aol.com...
Quoted message said:
>They may well have but the article gives no details at all about the technique used to measure
>vitamin E.
>
>MB
Well .. do some research ..
Maybe you will find out what they used ..
Maybe you will be able to ALSO use the same method ..
Then again .. maybe .. not ..
Half full or half empty ..
Quoted message said:
You've got the article Tom - you tell me.
You've .. got .. the .. kid ..
Quoted message said:
MB
"Cytoadhesion" - LOL !!!
Cut me some slack .. jack ..
Place the meaning .. here ..
Cytoadhesion -
Are you serious ?

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Manky Badger
Archived post

22 February 2004, 17:12 UTC #13

"doe" <[email hidden]> wrote in message
"]news:[email hidden]...
Quoted message said:
Quoted message said:
Subject: Re: vitamin E levels acts as a protective factor From: "Manky Badger"
[email hidden] Date: 2/21/2004 5:03 PM Mountain Standard Time Message-
id: <[email hidden]>
Quoted message said:
Quoted message said:
Place the meaning .. here ..
Cytoadhesion -
Are you serious ?
Yes ..
Not in my dictionary ..
Off the top of your head ..
Or .. your .. dictionary ..
In all seriousness Tom I really can't beleive that you don't understad this word. If you don't
understand something this basic (and I don't mean that rudely - it really is basic) then it must
cast considerable doubt over your ability to understand any of the articles that you keep posting.
But, to answer your question - the first lecture I give my trainees is one about the language &
terminology used in science. Break the words down to their components:
Cyto - appertaining to cells Adhesion - sticking
MB

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