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VAP A Better Cholesterol Test

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General fitness, health and nutrition
Published
7 June 2004
Last activity
12 June 2004
Original author
Robert
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  1. A newer testing, top of the line, next generation for lipids
    is being performed. Fasting is not necessary and more info
    is provided than before. This is an excerpt from the
    companies VP Athertech,Inc.

    The Vertical Auto Profile Cholesterol Test or VAP, is the
    only lipoprotein panel that directly measures all National
    Cholesterol Education Project (NCEP) Adult Treatment Panel
    III(ATPIII) primary target, LDL-Cholesterol, as well as both
    secondary treatment targets and all three ATPIII "emerging
    lipid risk factors,"in a single test. This information
    positions the VAP Cholesterol Test as a better, more
    efficient cholesterol test for all patients at risk for
    coronary artery disease, relative to the older lipid panel.
    There are several decades of medical evidence supporting the
    LDL-C and non-HDL-C therapy targets, the latter being a
    superior detector of risk in women. In addition the VAP
    Cholesterol Test serves as an early detector of the
    Metabolic Syndrome through identification of the
    dyslipidemia triad that presages onset of hyperglycemia.
    Finally ATPIII considers Lp(a), small/dense LDL and the
    Remnant Lipoprotein cholesterol as emerging lipid risk
    factors which may be counted as an additional risk factor in
    setting a lower LDL-C target. Presence of the emerging lipid
    risk factors also helps clinicians decide when combination
    lipid lowering therapy versus statin monotherapy will be
    required. For these reasons, and because the VAP optimizes
    compliance with the ATPIII guidelines, it is time to replace
    the ordinary lipid panel with the VAP test. The VAP method
    begins with non-equilibrium density gradient
    ultracentrifugation to separate all five major lipoproteins:
    HDL,Lp(a), R-LDL,IDL and VLDL, based on their size and
    density characteristics. R-LDL-C, or "real-LDL" is defined
    as the total LDL-C minus its Lp(a)-C and IDL-C components.
    Ultracentifiugal separation is followed by enzymatic
    determination of cholesterol in all lipoprotein fractions
    with approximately 400 sequential spectrophotometric
    measurements per sample. Thus, two key properties of all
    lipoproteins are determined both relative density and
    cholesterol concentration. The final stage of VAP analysis
    employs proprietary software deconvolution software to
    determine subfractions of HDL,LDL, and VLDL. All VAP testing
    is performed at Atherotech,INC, a CDC-NHLBI standardized
    cholesterol testing laboratory, which offers the VAP to
    other laboratories for well under US$50. The VAP is
    distributed by ARUP, LabCorp, the Mayo Clinic,
    QuestDiagnostics, and Specialty Labs.................
    ..................................

    Separate handout

    Diagnosis:

    Elevated LDL-R

    Elevated Lp(a)

    Elevated IDL

    LDL pattern Density shifts

    Elevated VLDL and TG

    Low HDL2

    Metabolic Syndrome

    Therapeutic lifestyle changes and drugs used for each
    category are included.

  2. This is a press release. What do you expect them to say?

    It sucks?

  3. Yeah, that would be handy if cholesterol levels actually
    meant anything useful.

    TC

    "Robert" <[email hidden]> wrote in message
    news:<[email hidden]>...

    Quoted message said:

    A newer testing, top of the line, next generation for
    lipids is being performed. Fasting is not necessary and
    more info is provided than before. This is an excerpt from
    the companies VP Athertech,Inc.

    The Vertical Auto Profile Cholesterol Test or VAP, is the
    only lipoprotein panel that directly measures all National
    Cholesterol Education Project (NCEP) Adult Treatment Panel
    III(ATPIII) primary target, LDL-Cholesterol, as well as
    both secondary treatment targets and all three ATPIII
    "emerging lipid risk factors,"in a single test. This
    information positions the VAP Cholesterol Test as a
    better, more efficient cholesterol test for all patients
    at risk for coronary artery disease, relative to the older
    lipid panel. There are several decades of medical evidence
    supporting the LDL-C and non-HDL-C therapy targets, the
    latter being a superior detector of risk in women. In
    addition the VAP Cholesterol Test serves as an early
    detector of the Metabolic Syndrome through identification
    of the dyslipidemia triad that presages onset of
    hyperglycemia. Finally ATPIII considers Lp(a), small/dense
    LDL and the Remnant Lipoprotein cholesterol as emerging
    lipid risk factors which may be counted as an additional
    risk factor in setting a lower LDL-C target. Presence of
    the emerging lipid risk factors also helps clinicians
    decide when combination lipid lowering therapy versus
    statin monotherapy will be required. For these reasons,
    and because the VAP optimizes compliance with the ATPIII
    guidelines, it is time to replace the ordinary lipid panel
    with the VAP test. The VAP method begins with non-
    equilibrium density gradient ultracentrifugation to
    separate all five major lipoproteins: HDL,Lp(a), R-LDL,IDL
    and VLDL, based on their size and density characteristics.
    R-LDL-C, or "real-LDL" is defined as the total LDL-C minus
    its Lp(a)-C and IDL-C components. Ultracentifiugal
    separation is followed by enzymatic determination of
    cholesterol in all lipoprotein fractions with
    approximately 400 sequential spectrophotometric
    measurements per sample. Thus, two key properties of all
    lipoproteins are determined both relative density and
    cholesterol concentration. The final stage of VAP analysis
    employs proprietary software deconvolution software to
    determine subfractions of HDL,LDL, and VLDL. All VAP
    testing is performed at Atherotech,INC, a CDC-NHLBI
    standardized cholesterol testing laboratory, which offers
    the VAP to other laboratories for well under US$50. The
    VAP is distributed by ARUP, LabCorp, the Mayo Clinic,
    QuestDiagnostics, and Specialty Labs.................
    ..................................

    Separate handout

    Diagnosis:

    Elevated LDL-R

    Elevated Lp(a)

    Elevated IDL

    LDL pattern Density shifts

    Elevated VLDL and TG

    Low HDL2

    Metabolic Syndrome

    Therapeutic lifestyle changes and drugs used for each
    category are included.

  4. "Jeff" <[email hidden]> wrote in message
    "]news:[email hidden]...

    Quoted message said:

    This is a press release. What do you expect them to say?

    It sucks?


    It's in clinical use already. There are situations where
    the standard cholesterol panel based on calculated LDL
    Friedewald formula is unacceptable. The alternative is to
    perform a measured LDL via enzymatic reactions. Any
    clinical trials involving insulin resistance subjects
    should employ directly measured LDL-C. The
    ultracentrifugation methodology is the reference method in
    which all the other tests are based on.

  5. [email hidden] (tcomeau) wrote in message news:<[email hidden]>...

    Quoted message said:

    Yeah, that would be handy if cholesterol levels actually
    meant anything useful.

    TC


    Yep. Just another massive waste of time and money in
    pursuit of a horribly flawed theory. I would be
    interested in knowing just how many HUNDREDS of millions
    of dollars have been wasted on the lipid
    hypothesis/cholesterol theory over the last 50 years or
    so. It is absolutely insane.

  6. "tcomeau" <[email hidden]> wrote in message
    "]news:[email hidden]...

    Quoted message said:

    Yeah, that would be handy if cholesterol levels actually
    meant anything


    useful.

    Quoted message said:


    TC

    It is the break down of the components of lipids and not
    simply cholesterol. It specifically quantifies these
    components based on high risk factors. These specific
    abnormalities may then be targeted by specific drugs.
    Statin, bile acid sequestrant, Niacin, Fenofibrate,
    Esterogen, Raloxifene, ASA, Fish oils, or Glitazones. If for
    example you have an elevated Lp(a) you would use Niacin,
    Fenofibrate, Esterogen, Raloxiene or and ASA. You don't get
    a Lp(a) level with the regular lipid panel. You have to ask
    for it separately. Statin drugs don't work for those who
    have a heart attack and have a benign looking regular lipid
    panel but have a high Lp(a). Those are the cases where you
    say cholesterol doesn't provide anything useful but again
    you have to look at all the high risk factors some of which
    are not included in the regular choelsterol testing.

    Quoted message said:


    "Robert" <[email hidden]> wrote in message


    news:<[email hidden]>...

    Quoted message said:
    Quoted message said:

    A newer testing, top of the line, next generation for
    lipids is being performed. Fasting is not necessary and
    more info is provided than


    before.

    Quoted message said:
    Quoted message said:

    This is an excerpt from the companies VP Athertech,Inc.

    The Vertical Auto Profile Cholesterol Test or VAP, is
    the only


    lipoprotein

    Quoted message said:
    Quoted message said:

    panel that directly measures all National Cholesterol
    Education Project (NCEP) Adult Treatment Panel
    III(ATPIII) primary target,


    LDL-Cholesterol, as

    Quoted message said:
    Quoted message said:

    well as both secondary treatment targets and all three
    ATPIII "emerging lipid risk factors,"in a single test.
    This information positions the VAP Cholesterol Test as
    a better, more efficient cholesterol test for all
    patients at risk for coronary artery disease, relative
    to the older


    lipid

    Quoted message said:
    Quoted message said:

    panel. There are several decades of medical evidence
    supporting the LDL-C and non-HDL-C therapy targets, the
    latter being a superior detector of risk


    in

    Quoted message said:
    Quoted message said:

    women. In addition the VAP Cholesterol Test serves as an
    early detector


    of

    Quoted message said:
    Quoted message said:

    the Metabolic Syndrome through identification of the
    dyslipidemia triad


    that

    Quoted message said:
    Quoted message said:

    presages onset of hyperglycemia. Finally ATPIII
    considers Lp(a),


    small/dense

    Quoted message said:
    Quoted message said:

    LDL and the Remnant Lipoprotein cholesterol as emerging
    lipid risk


    factors

    Quoted message said:
    Quoted message said:

    which may be counted as an additional risk factor in
    setting a lower


    LDL-C

    Quoted message said:
    Quoted message said:

    target. Presence of the emerging lipid risk factors
    also helps


    clinicians

    Quoted message said:
    Quoted message said:

    decide when combination lipid lowering therapy versus
    statin monotherapy will be required. For these reasons,
    and because the VAP optimizes compliance with the ATPIII
    guidelines, it is time to replace the


    ordinary

    Quoted message said:
    Quoted message said:

    lipid panel with the VAP test. The VAP method begins
    with non-equilibrium density gradient
    ultracentrifugation to separate all five major
    lipoproteins: HDL,Lp(a), R-LDL,IDL and VLDL, based on
    their size and density characteristics. R-LDL-C, or "real-
    LDL" is defined as the total LDL-C minus its Lp(a)-C


    and

    Quoted message said:
    Quoted message said:

    IDL-C components. Ultracentifiugal separation is
    followed by enzymatic determination of cholesterol in
    all lipoprotein fractions with approximately 400
    sequential spectrophotometric measurements per sample.
    Thus, two key properties of all lipoproteins are
    determined both


    relative

    Quoted message said:
    Quoted message said:

    density and cholesterol concentration. The final stage
    of VAP analysis employs proprietary software
    deconvolution software to determine subfractions of
    HDL,LDL, and VLDL. All VAP testing is performed at
    Atherotech,INC, a CDC-NHLBI standardized cholesterol
    testing laboratory, which offers the VAP to other
    laboratories for well under US$50. The VAP is
    distributed by ARUP, LabCorp, the Mayo Clinic,


    QuestDiagnostics,

    Quoted message said:
    Quoted message said:

    and Specialty Labs.................
    ..................................

    Separate handout

    Diagnosis:

    Elevated LDL-R

    Elevated Lp(a)

    Elevated IDL

    LDL pattern Density shifts

    Elevated VLDL and TG

    Low HDL2

    Metabolic Syndrome

    Therapeutic lifestyle changes and drugs used for each
    category are


    included.

  7. "Wolfbrother" <[email hidden]> wrote in message
    "]news:[email hidden]...

    Quoted message said:

    [email hidden] (tcomeau) wrote in message


    news:<[email hidden]>...

    Quoted message said:
    Quoted message said:

    Yeah, that would be handy if cholesterol levels actually
    meant anything


    useful.

    Quoted message said:
    Quoted message said:


    TC


    Yep. Just another massive waste of time and money in
    pursuit of a horribly flawed theory. I would be
    interested in knowing just how many HUNDREDS of
    millions of dollars have been wasted on the lipid
    hypothesis/cholesterol theory over the last 50 years
    or so. It is absolutely insane.

    You are very bright there Wolfbrother, can't fool you. Yeah,
    cholesterol has nothing to do with it and you can eat
    anything you want. People get heart attacks because of bad
    luck. There's nothing you can do to prevent them. All those
    millions could have been spent on good beer.

  8. "Robert" <[email hidden]> wrote in message news:<[email hidden]>...

    Quoted message said:

    "tcomeau" <[email hidden]> wrote in message
    "]news:[email hidden]...

    Quoted message said:

    Yeah, that would be handy if cholesterol levels actually
    meant anything


    useful.

    Quoted message said:


    TC

    It is the break down of the components of lipids and not
    simply cholesterol. It specifically quantifies these
    components based on high risk factors. These specific
    abnormalities may then be targeted by specific drugs.
    Statin, bile acid sequestrant, Niacin, Fenofibrate,
    Esterogen, Raloxifene, ASA, Fish oils, or Glitazones. If
    for example you have an elevated Lp(a) you would use
    Niacin, Fenofibrate, Esterogen, Raloxiene or and ASA. You
    don't get a Lp(a) level with the regular lipid panel. You
    have to ask for it separately. Statin drugs don't work for
    those who have a heart attack and have a benign looking
    regular lipid panel but have a high Lp(a). Those are the
    cases where you say cholesterol doesn't provide anything
    useful but again you have to look at all the high risk
    factors some of which are not included in the regular
    choelsterol testing.

    A large number of people who die of heart disease never had
    problems with cholesterol. And a large number of people with
    elevated cholesterol levels do not suffer from heart
    disease. Cholesterol and blood lipid profile are transient,
    they change thruout the day with meals, with physical
    activity, with inactivity and with sleep periods, so to take
    one snapshot of ones lipid profile and base treatment on
    that is just plain ridiculous.

    Good dietary fat, contrary to popular belief, improves blood
    lipid profiles. Increased dietary cholesterol does not
    equate to increased blood cholesterol levels. The opposite
    is true. The whole high cholesterol scare [censored] is
    ridiculous.

    There is a much higher correlation with homocysteine
    levels and heart disease than there is with cholesterol
    and heart disease. This points to general malnutrition as
    the major causative factor in heart disease. B vitamins
    are needed to keep the homocysteine levels down. Refined
    carbs deplete B vitamins.

    TC

  9. "tcomeau" <[email hidden]> wrote in message
    "]news:[email hidden]...

    Quoted message said:

    "Robert" <[email hidden]> wrote in message


    news:<[email hidden]>...

    Quoted message said:
    Quoted message said:

    "tcomeau" <[email hidden]> wrote in message
    "]news:[email hidden]...

    Quoted message said:

    Yeah, that would be handy if cholesterol levels
    actually meant


    anything

    Quoted message said:
    Quoted message said:

    useful.

    Quoted message said:


    TC

    It is the break down of the components of lipids and not
    simply


    cholesterol.

    Quoted message said:
    Quoted message said:

    It specifically quantifies these components based on
    high risk factors. These specific abnormalities may then
    be targeted by specific drugs.


    Statin,

    Quoted message said:
    Quoted message said:

    bile acid sequestrant, Niacin, Fenofibrate, Esterogen,
    Raloxifene, ASA,


    Fish

    Quoted message said:
    Quoted message said:

    oils, or Glitazones. If for example you have an elevated
    Lp(a) you would use Niacin,


    Fenofibrate,

    Quoted message said:
    Quoted message said:

    Esterogen, Raloxiene or and ASA. You don't get a Lp(a)
    level with the regular lipid panel. You have to ask for
    it separately. Statin drugs


    don't

    Quoted message said:
    Quoted message said:

    work for those who have a heart attack and have a benign
    looking regular lipid panel but have a high Lp(a). Those
    are the cases where you say cholesterol doesn't provide
    anything useful but again you have to look


    at

    Quoted message said:
    Quoted message said:

    all the high risk factors some of which are not included
    in the regular choelsterol testing.

    A large number of people who die of heart disease never
    had problems with cholesterol. And a large number of
    people with elevated cholesterol levels do not suffer from
    heart disease.

    That is true with the old methodology of simply reporting
    total cholesterol, LDL, and HDL by conventional means. In
    the Framingham Heart Study Lp(a) was shown to double
    coronary events. Thus on a mg/dl of cholesterol basis it is
    10 times as atherogenic as R-LDL-C. It is not rare and it is
    inherited in an autosomal dominant fashion. Lp(a) is
    elevated in 1 in 4 persons. Lp(a) is unaffected by statins
    and is best lowered with niacin and aspirin. You don't pick
    up these people with the old lipid panels. All the newer
    studies HATS, the Heart Protection Study, PEPI and many
    others did not simply use the old methodology testing. They
    are well aware of the flaws of simply measuring cholesterol.

    Cholesterol and

    Quoted message said:

    blood lipid profile are transient, they change thruout the
    day with meals, with physical activity, with inactivity
    and with sleep periods, so to take one snapshot of ones
    lipid profile and base treatment on that is just plain
    ridiculous.

    All laboratory parameters for anything that you measure
    changes. Glucose, hormones, and even white countes change
    depending of the time of day, fasting or not, position in
    which blood was drawn, amount of excerise or not so that is
    a poor arguement. You look for patterns and associations of
    relative markers and not simply changes in one lab test.
    Detection of insulin resisteance with high specificity can
    be made with seeing the triad of small/dense LDL-C,
    elevated TG's and low HDL2. HDL2 is the lipoprotein
    responsible for "reverse cholesterol transport" and is the
    component of HDL raised most by excercise. Total LDL-C has
    no correlation with the degree of insulin resistance while
    having that triad is very highly associated with insulin
    resistance. You also look for genetic risk factors that are
    constant no matter what time of day or under what
    conditions the blood is drawn.

    Quoted message said:


    Good dietary fat, contrary to popular belief, improves
    blood lipid profiles. Increased dietary cholesterol does
    not equate to increased blood cholesterol levels. The
    opposite is true. The whole high cholesterol scare
    [censored] is ridiculous.


    You are welcome to eat anything you want but you also need
    to have your blood evaluated to detect diagnostic patterns
    to see exactly where you stand and not rely on your
    subjective beliefs. If you have Metabolic Syndrome then
    eating much fat is not good for you and excercise is also
    recommended.

    Quoted message said:


    There is a much higher correlation with homocysteine
    levels and heart disease than there is with cholesterol
    and heart disease. This points to general malnutrition as
    the major causative factor in heart disease. B vitamins
    are needed to keep the homocysteine levels down. Refined
    carbs deplete B vitamins.

    TC

    Wrong attitude that I see every day by everyone here and you
    are no different. They always seek to find the "most "
    higher correlation. If you get a heart attack do you expect
    your doctor to perform a homocysteine and if it turns out
    normal do you expect him to stop the diagnostic workup?
    There is rarely just one cause for any disease in the
    general population. You want to find a specific cause within
    any given individual and you have to check for all known
    causes. From experience on performing many of the above I
    rarely see a high homocysteine levels and I have seen many,
    many ,heart attack patients.

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