General fitness, health and nutrition · Public discussion

Quercetin treatment reverses cognitive deficits

Doe · 2004-01-06T15:19:32+00:00

Free Radic Res. 2003 Nov;37(11):1245-52. Links Reversal of aging and chronic ethanol-induced cognitive dysfunction by quercetin a bioflavonoid. Singh A, Naidu PS, Kulkarni SK. Pharmacology Division, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh- 160014, India. Cognitive dysfunction, one of the most striking age-related impairments seen in human beings, has been correlated to the vulnerability of the brain to increased oxidative stress during aging process. Quercetin is a bioflavonoid with strong antioxidant properties. Experiments were performed to study the possible effects of quercetin on cognitive performance of young, aged or ethanol- intoxicated mice (an animal model for cognition dysfunction) using one trail step down type of passive avoidance and elevated plus maze tasks, respectively. Aged or chronic ethanol-treated mice showed poor retention of memory in step-down passive avoidance and in elevated plus-maze task. Chronic administration of quercetin (10, 25 and 50 mg/kg) for 30 days or its co-administration with ethanol (15% w/v, 2g/kg per orally) for 24 days significantly reversed the age-related or chronic ethanol-induced retention deficits in both the test paradigms. However, in both memory paradigms chronic administration of quercetin failed to modulate the retention performance of young mice. Chronic quercetin administration for 30 days also reversed age associated increase in TBARS levels and decline in forebrain total glutathione (GSH), SOD and catalase levels. Chronic ethanol administration to young mice produced an increase in lipid peroxidation, and a decline in forebrain total glutathione (GSH), SOD and catalase levels, which was significantly reversed by the co- administration of quercetin (10, 25 and 50 mg/kg). The results of the present study showed that chronic quercetin treatment reverses cognitive deficits in aged and ethanol-intoxicated mice, which is associated with its antioxidant property. PMID: 14703737 [PubMed - in process] -------------------------------------------------------------------------- ------ Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://pages.ivillage.com/ironjustice/manisaherbivore DEAD PEOPLE WALKING http://pages.ivillage.com/ironjustice/deadpeoplewalking

Started by Doe · 6 January 2004 · Last activity 14 January 2004 · 3 posts · 401 views

← Back to General fitness, health and nutrition

This thread is locked and is currently read-only.

Thread details

What we know about this thread

Original section: General fitness, health and nutrition
Published: 6 January 2004
Last activity: 14 January 2004
Original author: Doe
Posts: 3
Discussion status: Public discussion
Total views: 401
Views / 30 days: 0

The navigation and discussion metadata provide context. Posts remain in their original chronological order.

Original post
Doe
Archived post

6 January 2004, 15:19 UTC #1

Free Radic Res. 2003 Nov;37(11):1245-52. Links
Reversal of aging and chronic ethanol-induced cognitive dysfunction by quercetin a bioflavonoid.
Singh A, Naidu PS, Kulkarni SK.
Pharmacology Division, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh-
160014, India.
Cognitive dysfunction, one of the most striking age-related impairments seen in human beings, has
been correlated to the vulnerability of the brain to increased oxidative stress during aging
process. Quercetin is a bioflavonoid with strong antioxidant properties. Experiments were performed
to study the possible effects of quercetin on cognitive performance of young, aged or ethanol-
intoxicated mice (an animal model for cognition dysfunction) using one trail step down type of
passive avoidance and elevated plus maze tasks, respectively. Aged or chronic ethanol-treated mice
showed poor retention of memory in step-down passive avoidance and in elevated plus-maze task.
Chronic administration of quercetin (10, 25 and 50 mg/kg) for 30 days or its co-administration with
ethanol (15% w/v, 2g/kg per orally) for 24 days significantly reversed the age-related or chronic
ethanol-induced retention deficits in both the test paradigms. However, in both memory paradigms
chronic administration of quercetin failed to modulate the retention performance of young mice.
Chronic quercetin administration for 30 days also reversed age associated increase in TBARS levels
and decline in forebrain total glutathione (GSH), SOD and catalase levels. Chronic ethanol
administration to young mice produced an increase in lipid peroxidation, and a decline in forebrain
total glutathione (GSH), SOD and catalase levels, which was significantly reversed by the co-
administration of quercetin (10, 25 and 50 mg/kg). The results of the present study showed that
chronic quercetin treatment reverses cognitive deficits in aged and ethanol-intoxicated mice, which
is associated with its antioxidant property.
PMID: 14703737 [PubMed - in process]
--------------------------------------------------------------------------
------
Who loves ya. Tom Jesus Was A Vegetarian! jesuswasavegetarian.7h.comjesuswasavegetarian.7h.com Man Is A Herbivore!
pages.ivillage.commanisaherbivore DEAD PEOPLE WALKING
pages.ivillage.comdeadpeoplewalking

Report
Doe
Archived post

6 January 2004, 15:19 UTC #2

Quoted message said:
Subject: Quercetin treatment reverses cognitive deficits
Free Radic Biol Med. 2002 Jul 1;33(1):63-70. elsevier.nlindex.htt
Protection against oxidative damage of erythrocyte membrane by the flavonoid quercetin and its
relation to iron chelating activity
--------------------------------------------------------------------------
------------------------------------
Quercetin metabolism in the lens: role in inhibition of hydrogen peroxide induced cataract.
Cornish KM, Williamson G, Sanderson J.
School of Biological Sciences, University of East Anglia, Norwich, Norfolk, UK.
Oxidative stress is implicated in the initiation of maturity onset cataract. Quercetin, a major
flavonol in the diet, inhibits lens opacification in a lens organ culture oxidative model of
cataract. The aim of this research was to investigate the metabolism of quercetin in the lens and
show how its metabolism affects the ability to prevent oxidation-induced opacity. The LOCH model
(Free Radical Biology & Medicine 26:639; 1999) was employed, using rat lenses to investigate the
effects of quercetin and metabolites on hydrogen peroxide-induced opacification. High-performance
liquid chromatography analysis showed that the intact rat lens is capable of converting quercetin
aglycone to 3'-O-methyl quercetin (isorhamnetin). Over a 6 h culture period no further metabolism of
the 3'-O-methyl quercetin occurred. Loss of quercetin in the lens was accounted for by the increase
in 3'-O-methyl quercetin. Incubation with 3,5-dinitrocatechol (10 microM), a catechol-O-
methyltransferase (COMT) inhibitor, prevented the conversion of quercetin to 3'-O- methyl quercetin.
The presence of both membrane-bound and soluble COMT was confirmed by immunoblotting. The results
demonstrate that in the rat lens COMT methylates quercetin and that the product accumulates within
the lens. Quercetin (10 microM) and 3'-O-methyl quercetin (10 microM) both inhibited hydrogen peroxide-
(500 microM) induced sodium and calcium influx and lens opacification. Incubation of lenses with
quercetin in the presence of COMT inhibitor revealed that the efficacy of quercetin is not dependent
on its metabolism to 3'-O-methyl quercetin. The results indicate dietary quercetin and metabolites
are active in inhibiting oxidative damage in the lens and thus could play a role in prevention of
cataract formation.
PMID: 12086683 [PubMed - indexed for MEDLINE
Who loves ya. Tom
Jesus Was A Vegetarian! jesuswasavegetarian.7h.comjesuswasavegetarian.7h.com Man Is A Herbivore!
pages.ivillage.commanisaherbivore DEAD PEOPLE WALKING
pages.ivillage.comdeadpeoplewalking

Report
William A. Noye
Archived post

14 January 2004, 06:24 UTC #3

I thought it inhibited the formation of cataracts by inhibiting aldol reductase and thereby reducing
sorbitol levels in the lens.
"doe" <[email hidden]> wrote in message "]news:[email hidden]...
Quoted message said:
Quoted message said:
Subject: Quercetin treatment reverses cognitive deficits
Free Radic Biol Med. 2002 Jul 1;33(1):63-70. elsevier.nlindex.htt
Protection against oxidative damage of erythrocyte membrane by the

flavonoid
Quoted message said:
quercetin and its relation to iron chelating activity
--------------------------------------------------------------------------
------------------------------------
Quercetin metabolism in the lens: role in inhibition of hydrogen peroxide induced cataract.
Cornish KM, Williamson G, Sanderson J.
School of Biological Sciences, University of East Anglia, Norwich, Norfolk, UK.
Oxidative stress is implicated in the initiation of maturity onset cataract. Quercetin, a major
flavonol in the diet, inhibits lens opacification in a lens organ culture oxidative model of
cataract. The aim of this research was to investigate the metabolism of quercetin in the lens and
show how its metabolism affects the ability to prevent oxidation-induced opacity. The LOCH model
(Free Radical Biology & Medicine 26:639; 1999) was employed, using rat lenses to investigate the
effects of quercetin and metabolites on hydrogen peroxide-induced opacification. High-performance
liquid chromatography analysis showed that the intact rat lens is capable of converting quercetin
aglycone to 3'-O-methyl quercetin (isorhamnetin). Over a 6 h culture period no further metabolism
of the 3'-O-methyl quercetin occurred. Loss of quercetin in the lens was accounted for by the
increase in 3'-O-methyl quercetin. Incubation with 3,5-dinitrocatechol (10 microM), a catechol-O-
methyltransferase (COMT) inhibitor, prevented the conversion of quercetin to 3'-O- methyl
quercetin. The presence of both membrane-bound and soluble COMT was confirmed by immunoblotting.
The results demonstrate that in the rat lens COMT methylates quercetin and that the product
accumulates within the lens. Quercetin (10 microM) and 3'-O-methyl quercetin (10 microM) both
inhibited hydrogen peroxide- (500 microM) induced sodium and calcium influx and lens
opacification. Incubation of lenses with quercetin in the presence of COMT inhibitor revealed that
the efficacy of quercetin is not dependent on its metabolism to 3'-O-methyl quercetin. The results
indicate dietary quercetin and metabolites are active in inhibiting oxidative damage in the lens
and thus could play a role in prevention of cataract formation.
PMID: 12086683 [PubMed - indexed for MEDLINE
Who loves ya. Tom
Jesus Was A Vegetarian! jesuswasavegetarian.7h.comjesuswasavegetarian.7h.com Man Is A Herbivore!
pages.ivillage.commanisaherbivore DEAD PEOPLE WALKING

pages.ivillage.comdeadpeoplewalking

Report

Active in the last 60 minutes

Active in this thread

0 users · 0 guests ·0 bots ·0 total

No signed-in users are active right now.

No known search crawlers active right now.

What we know about this thread

Continue through CyclingForums

Active in this thread