General fitness, health and nutrition · Public discussion

Nasunin Chelates Iron

Email address hidden · 2007-04-30T13:55:18+00:00

Noda Y, Kaneyuki T, Igarashi K, Mori A, Packer L Antioxidant activity of nasunin, an anthocyanin in eggplant. [Journal Article] Res Commun Mol Pathol Pharmacol 1998 Nov; 102(2) :175-87. Delphinidine-3-(p-coumaroylrutinoside)-5-glucoside (nasunin), an anthocyanin was isolated as purple colored crystals from eggplant peels, Solanum melongena L. 'Chouja'. Using an electron spin resonance spectrometer and 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), spin trapping, hydroxyl (.OH) or superoxide anion radicals (02*-) generated by the Fenton reaction or the hypoxanthine-xanthine oxidase system were measured as DMPO-OH or DMPO-OOH spin adducts. L-Ascorbic acid 2- [3,4-dihydro-2,5,7,8-tetra-methyl-2-(4,8,12-trimethyltridecyl)-2 H-1- benzopyran-6yl-hydrogen phosphate] potassium salt (EPC-K1) and bovine erythrocyte superoxide dismutase (SOD) were used as standards for .OH and O2*-, respectively. Nasunin directly scavenged O2*- with a potency of 143+/-8 SOD-equivalent units/mg), and inhibited formation of DMPO- OH (0.65+/-0.07 EPC-K1 micromol/mg). A spectrophotometric study showed that nasunin formed an iron complex with a molar ratio of nasunin : Fe3+ of 2 : 1. Therefore, hydroxyl radical scavenging by nasunin is not due to direct radical scavenging but inhibition of .OH generation by chelating iron. Nasunin (1 microM) significantly protected against lipid peroxidation of brain homogenates (p<0.001) as measured by malonaldehyde and 4-hydroxyalkenals. These findings demonstrate that nasunin is a potent O2*- scavenger and iron chelator which can protect against lipid peroxidation. ---------------------------------------------------------------------------- Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://tinyurl.com/a3cc3 DEAD PEOPLE WALKING http://tinyurl.com/zk9fk

Started by Email address hidden · 30 April 2007 · Last activity 30 April 2007 · 5 posts · 150 views

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Original section: General fitness, health and nutrition
Published: 30 April 2007
Last activity: 30 April 2007
Original author: Email address hidden
Posts: 5
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Original post
Email address hidden
Archived post

30 April 2007, 13:55 UTC #1

Noda Y, Kaneyuki T, Igarashi K, Mori A, Packer L
Antioxidant activity of nasunin, an anthocyanin in eggplant. [Journal
Article]
Res Commun Mol Pathol Pharmacol 1998 Nov; 102(2) :175-87.
Delphinidine-3-(p-coumaroylrutinoside)-5-glucoside (nasunin), an
anthocyanin was isolated as purple colored crystals from eggplant
peels, Solanum melongena L. 'Chouja'. Using an electron spin resonance
spectrometer and 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), spin
trapping, hydroxyl (.OH) or superoxide anion radicals (02*-) generated
by the Fenton reaction or the hypoxanthine-xanthine oxidase system
were measured as DMPO-OH or DMPO-OOH spin adducts. L-Ascorbic acid 2-
[3,4-dihydro-2,5,7,8-tetra-methyl-2-(4,8,12-trimethyltridecyl)-2 H-1-
benzopyran-6yl-hydrogen phosphate] potassium salt (EPC-K1) and bovine
erythrocyte superoxide dismutase (SOD) were used as standards for .OH
and O2*-, respectively. Nasunin directly scavenged O2*- with a potency
of 143+/-8 SOD-equivalent units/mg), and inhibited formation of DMPO-
OH (0.65+/-0.07 EPC-K1 micromol/mg). A spectrophotometric study showed
that nasunin formed an iron complex with a molar ratio of nasunin :
Fe3+ of 2 : 1. Therefore, hydroxyl radical scavenging by nasunin is
not due to direct radical scavenging but inhibition of .OH generation
by chelating iron. Nasunin (1 microM) significantly protected against
lipid peroxidation of brain homogenates (p<0.001) as measured by
malonaldehyde and 4-hydroxyalkenals. These findings demonstrate that
nasunin is a potent O2*- scavenger and iron chelator which can protect
against lipid peroxidation.
----------------------------------------------------------------------------
Who loves ya.
Tom
Jesus Was A Vegetarian!
http://jesuswasavegetarian.7h.com
Man Is A Herbivore!
http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

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Email address hidden
Archived post

30 April 2007, 14:13 UTC #2

I wonder if there are any of those .. purple .. crystals in this ..
purple .. stuff .. ?
Remember .. 'anemia' .. is relative to .. **intelligence** .. OF ..
medical professionals.
Such as anemic women don't have diabetic dead babies.
http://www.sciencedaily.com/releases/2002/12/021213062321.htm
"Too Much Grape Juice Could Cause Iron Deficiency
The same antioxidant compounds in dark grape juice that are noted
for
their health benefits in fighting heart disease may have a
downside,
according to new research in the Journal of Agricultural and Food
Chemistry. In cell studies, scientists with the U.S. Department of
Agriculture and Cornell University found that polyphenols in
purple
(also called red) grape juice can inhibit the uptake of iron,
which
could increase the risk of iron-deficiency anemia."
--------------------------------------------------------------------------
http://abcnews.go.com/Health/story?id=2959851&page=1
Grape and Berry Juices: Elixers for Long Life?
Grape Juice-Industry-Funded Study Finds They May Help Prevent
Alzheimer's, Heart Disease By AMMU KANNAMPILLY
Mar. 17, 2007
Americans drink half the world's orange juice -- 21 quarts per person
each year. Most of them do so because of reasons connected to taste
and to the perceived health benefits of a glass of O.J. every day.
But that may change soon. According to a new study by scientists at
the University of Glasgow in Scotland, purple grape juice is now your
best bet for preventing heart disease, Alzheimer's disease and a host
of other chronic ailments.
Who loves ya.
Tom
Jesus Was A Vegetarian!
http://jesuswasavegetarian.7h.com
Man Is A Herbivore!
http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk

Report
Joan Carter
Archived post

30 April 2007, 15:58 UTC #3

email hidden said:
Delphinidine-3-(p-coumaroylrutinoside)-5-glucoside (nasunin), an
anthocyanin was isolated as purple colored crystals from eggplant
peels, Solanum melongena L. 'Chouja'. Using an electron spin resonance
Peckerhead!

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Sylv
Archived post

30 April 2007, 21:35 UTC #4

Joan;
Quoted message said:
On 30 Apr 2007 06:55:18 -0700, "[email hidden]"
Quoted message said:
Delphinidine-3-(p-coumaroylrutinoside)-5-glucoside (nasunin), an
anthocyanin was isolated as purple colored crystals from eggplant
peels, Solanum melongena L. 'Chouja'. Using an electron spin resonance
Peckerhead!
LOL! I'm gonna steal this epithet for Tommy!
Sylvia (still giggling!)

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Sylv
Archived post

30 April 2007, 21:47 UTC #5

Quoted post said:
Remember .. 'anemia' .. is relative to .. **intelligence** .. OF ..
medical professionals.
So that's why you're so stupid; too much bloodletting has made you
anemic. And dumb as a box of rocks.
Quoted message said:
Such as anemic women don't have diabetic dead babies.
This may qualify as the most moronic thing you have ever written.
Sylvia

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