Road Cycling · Public discussion

Testing for EPO.

Started by D. Ferguson · · Last activity · 3 posts · 568 views

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Road Cycling
Published
27 August 2005
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28 August 2005
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D. Ferguson
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  1. EPO is naturally occuring substance in the body so just it's presence
    doesn't say much. I know they test for quantities of specific proteins
    in the urine to try to quantify the amount of EPO in the body. Too
    many of these protiens and you are considered positive for EPO. But if
    your numbers are under a certain percentage then you are considered
    clean.

    So my question is this, how could high percentages of proteins last
    for 6 years? Were the samples frozen or just refrigerated?

    Surely that has to be paramount to the accuracy of the test. The
    degradation of these "basic isoforms" would certainly occur over time.
    Anyone familiar with the specifics on that?

    Something has to change over 6 years so do they allow for that based
    on storage?

    There seemed to be lot left out of the story other than that they are
    experimenting.

  2. D. Ferguson said:


    Surely that has to be paramount to the accuracy of the test. The
    degradation of these "basic isoforms" would certainly occur over time.

    Perhaps, but the test isn't based on the number, it's based on the
    proportion.
    http://www.wada-ama.org/rtecontent/document/td2004epo_en.pdf

  3. Robert Chung said:
    D. Ferguson said:


    Surely that has to be paramount to the accuracy of the test. The
    degradation of these "basic isoforms" would certainly occur over time.

    Perhaps, but the test isn't based on the number, it's based on the
    proportion.
    http://www.wada-ama.org/rtecontent/document/td2004epo_en.pdf

    Thanks for the link.

    The only thing I can really say to that thing is Holy [censored]!
    Is that the science that these guys careers are riding on?
    I've spent enough time in a lab (maybe 20 credit hours worth over the
    years) to know that the "Sample Preparation" comments alone would
    scare the [censored] out of me as a racer.

    No wonder the think you need at least 80% to have a positive, when
    anything over 40% would be above the normal range.

    "...any change to sample preparation may affect the isoform
    distribution..."

    That gives added meaning to "need a B sample to confirm".

    D

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