TGF-beta(1) / free radicals / iron

"asbestos,", nuff said, ignoring/unable to comprehend
difference between cause and effect.

Quoted message said:

Lab Invest. 2004 May 3 [Epub ahead of print] Related
Articles, Links

Asbestos-derived reactive oxygen species activate TGF-
beta(1).

Pociask DA, Sime PJ, Brody AR.

1Lung Biology Program, Department of Pathology, Tulane
University Health Science Center, New Orleans, LA, USA.

Transforming growth factor-beta(1) (TGF-beta(1)) is a
potent peptide that inhibits epithelial and mesenchymal
cell proliferation and stimulates the synthesis of
extracellular matrix components. This cytokine is produced
in a biologically latent complex bound to a latent-
associated peptide (LAP), and it is the disassociation of
this complex that regulates TGF-beta activity. A number of
mechanisms have been shown to activate TGF-beta(1). We show
here that reactive oxygen species (ROS), generated by the
iron in chrysotile or crocidolite asbestos, mediate the
biological activity of TGF-beta(1). Recombinant human
latent TGF-beta(1) was activated in a cell free system in
the presence of asbestos and ascorbic acid. Latent TGF-
beta(1) was overexpressed in both A549 and mink lung
epithelial cell lines through an adenovirus vector
containing the full-length construct for porcine TGF-
beta(1). This latent TGF-beta(1) was activated in a concentration-
dependant fashion by introducing asbestos into the cell
cultures. This activation was reduced significantly through
the use of superoxide dismutase, catalase or deferoxamine.
Amino-acid constituents of the LAP were oxidized as
demonstrated by the appearance of carbonyls detected by
Western analysis. The oxidized LAP could no longer form a
complex with TGF-beta(1). Our data support the postulate
that ROS derived from asbestos provide a mechanism for
activating TGF-beta(1) in the alveolar environment by
oxidizing amino acids in LAP.Laboratory Investigation
advance online publication, 3 May 2004;
doi:10.1038/labinvest.3700109

PMID: 15122306 [PubMed - as supplied by publisher]

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Who loves ya. Tom

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Quoted message said:

Subject: Re: TGF-beta(1) / free radicals / iron
From: [email hidden]
Date: 5/5/2004 7:44 AM Mountain Daylight Time
Message-id: <[email hidden]>

"asbestos,", nuff said, ignoring/unable to comprehend
difference between cause and effect.

You are stupid .. will forever BE .. stupid ..

That is self .. evident ..

How does .. that .. feel ..

To .. BE .. stupid ..

<<snip>> We show here that reactive oxygen species (ROS),
generated by the IRON in chrysotile or crocidolite asbestos,
mediate the biological activity of TGF-beta(1). <<snip>>

Read to fkg comprehend you stupid .. jrkff ..

Quoted message said:

Quoted message said:

Lab Invest. 2004 May 3 [Epub ahead of print] Related
Articles, Links

Asbestos-derived reactive oxygen species activate TGF-
beta(1).

Pociask DA, Sime PJ, Brody AR.

1Lung Biology Program, Department of Pathology, Tulane
University Health Science Center, New Orleans, LA, USA.

Transforming growth factor-beta(1) (TGF-beta(1)) is a
potent peptide that inhibits epithelial and mesenchymal
cell proliferation and stimulates the synthesis of
extracellular matrix components. This cytokine is produced
in a biologically latent complex bound to a latent-
associated peptide (LAP), and

it

Quoted message said:

is the disassociation of this complex that regulates TGF-
beta activity. A number of mechanisms have been shown to
activate TGF-beta(1). We show here

that

Quoted message said:

reactive oxygen species (ROS), generated by the iron in
chrysotile or crocidolite asbestos, mediate the biological
activity of TGF-beta(1). Recombinant human latent TGF-
beta(1) was activated in a cell free system in

the

Quoted message said:

presence of asbestos and ascorbic acid. Latent TGF-beta(1)
was overexpressed

in

Quoted message said:

both A549 and mink lung epithelial cell lines through an
adenovirus vector containing the full-length construct for
porcine TGF-beta(1). This latent TGF-beta(1) was activated
in a concentration-dependant fashion by

introducing

Quoted message said:

asbestos into the cell cultures. This activation was
reduced significantly through the use of superoxide
dismutase, catalase or deferoxamine.

Amino-acid

Quoted message said:

constituents of the LAP were oxidized as demonstrated by
the appearance of carbonyls detected by Western analysis.
The oxidized LAP could no longer

form a

Quoted message said:

complex with TGF-beta(1). Our data support the postulate
that ROS derived

from

Quoted message said:

asbestos provide a mechanism for activating TGF-beta(1) in
the alveolar environment by oxidizing amino acids in
LAP.Laboratory Investigation advance online publication, 3
May 2004; doi:10.1038/labinvest.3700109

PMID: 15122306 [PubMed - as supplied by publisher]

----------------------------------------------------------
----------------
------

Who loves ya. Tom

Jesus Was A Vegetarian! jesuswasavegetarian.7h.comjesuswasavegetarian.7h.comOpen ↗
Man Is A Herbivore!
pages.ivillage.commanisaherbivoreOpen ↗ DEAD
PEOPLE WALKING
pages.ivillage.comdeadpeoplewalkingOpen ↗