General fitness, health and nutrition · Public discussion

Resveratrol conjugation on absorption

Started by Arbor · · Last activity · 2 posts · 419 views

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General fitness, health and nutrition
Published
23 July 2004
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24 July 2004
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Arbor
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  1. Darryl,
    You wrote in another post:

    Quoted message said:

    Doesn't matter which has higher levels of resveratrol since it's
    conjugated on absorption. I am unawares of any study that looks at
    the benefits, or lack thereof, of these conjugates. ;-)

    Did you have this abstract in mind?

    Arbi

    Clin Biochem. 2003 Feb;36(1):79-87. Related Articles, Links

    Absorption of three wine-related polyphenols in three different
    matrices by healthy subjects.

    Goldberg DM, Yan J, Soleas GJ.

    Department of Laboratory Medicine and Pathobiology, University of
    Toronto, ON, Canada M5G 1L5. [email hidden]

    BACKGROUND: Despite their powerful biologic activities conducive to
    protection against atherosclerosis, cancer and inflammatory diseases
    demonstrated in vitro, there is considerable doubt whether the
    polyphenolic constituents present in red wine and other dietary
    components are effective in vivo. OBJECTIVE: We have tested the
    absorptive efficiency of three of these constituents
    (trans-resveratrol, [+]-catechin and quercetin) when given orally to
    healthy human subjects in three different media. DESIGN: Twelve
    healthy males aged 25 to 45 were randomly assigned to three different
    groups consuming orally one of the following polyphenols:
    trans-resveratrol, 25 mg/70 kg; [+]-catechin 25 mg/70 kg; quercetin 10
    mg/70 kg. Each polyphenol was randomly administered at 4-week
    intervals in three different matrices: white wine (11.5% ethanol),
    grape juice, and vegetable juice/homogenate. Blood was collected at
    zero time and at four intervals over the first four hours after
    consumption; urine was collected at zero time and for the following
    24-h. The sums of free and conjugated polyphenols were measured in
    blood serum and urine by a gas-chromatographic method. RESULTS: All
    three polyphenols were present in serum and urine predominantly as
    glucuronide and sulfate conjugates, reaching peak concentrations in
    the former around 30-min after consumption. The free polyphenols
    accounted for 1.7 to 1.9% (trans-resveratrol), 1.1 to 6.5%
    ([+]-catechin) and 17.2 to 26.9% (quercetin) of the peak serum
    concentrations. The absorption of trans-resveratrol was the most
    efficient as judged by peak serum concentration, area-under-the curve
    (4 h) and urinary 24-h excretion (16-17% of dose consumed).
    [+]-Catechin was the poorest by these criteria (urine 24-h excretion
    1.2%-3.0% of dose consumed), with quercetin being intermediate (urine
    24-h excretion 2.9%-7.0% of dose consumed). Some significant matrix
    effects were observed for the serum polyphenol concentrations, but in
    the case of urine no matrix promoted significantly higher excretion
    than the other two. CONCLUSIONS: The absorption of these three
    polyphenols is broadly equivalent in aqueous and alcoholic matrices
    but, at peak concentrations of 10 to 40 nmol/L, is inadequate to
    permit circulating concentrations of 5 to 100 micromol/L consistent
    with in vitro biologic activity. The voluminous literature reporting
    powerful in vitro anticancer and antiinflammatory effects of the free
    polyphenols is irrelevant, given that they are absorbed as conjugates.

    PMID: 12554065 [PubMed - indexed for MEDLINE]

  2. Quoted message said:
    Quoted message said:

    Doesn't matter which has higher levels of resveratrol since it's
    conjugated on absorption. I am unawares of any study that looks at
    the benefits, or lack thereof, of these conjugates. ;-)

    Did you have this abstract in mind?

    Yeppers (I mentioned it later in the previous thread). Interestingly,
    the article briefly addresses de-alcoholized red wine (DRW) extracts
    vs. red wine...

    "Some recent investigations have generated data attempting to show
    that DRW components have biologic effects _in vivo_ independent of
    those of ethanol, but the results have not been convincing..."

    Quoted message said:

    Clin Biochem. 2003 Feb;36(1):79-87.

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