General fitness, health and nutrition · Public discussion

Purine World?

Started by TomHendricks474 · · Last activity · 4 posts · 576 views

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General fitness, health and nutrition
Published
18 January 2004
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21 January 2004
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TomHendricks474
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  1. More and more I'm wondering if before the RNA world there might have been a purine world - A and G.

    Those bases are the most common in Miller type experiments.

    Purines don't get dimers like pyrimidines - therefore I would think they would be safe under the UV
    onslaught.

    A RNA strand need not h-bond with complimentary bases. It could be all G, A, and assorted other
    bases (I,U,C etc, in small amounts). When jumbled into a pile there would certainly be some h-
    bonding among the bases.

    And now to the interesting part. Let's say the strand is folded with two naked ends sticking out. If
    those 2 ends are A or G, then it is likely we might have this type of coding: GA_, or GG_ Note the
    center base is the key one and the rest are the more stable G with 3 h-bonds.

    Also note that the N or _ base is wobble position. It may well have been that base farthest from the
    folded part of the strand - and the most shakey.

    GG_ is glycine - and with glycine we have no chirality problem. It is non polar

    GA_ is glatamic acid or aspartic acid - both acidic.

    Later GU_ would bring in basic aa's. Note there is no C needed yet.

    Now IF, and chemists help me if you can, IF that loose end middle base could h-bond to the carboxyl
    end of a peptide bond, I think we might have something here.

    A purine world that connects an RNA strand's loose ends with peptide bonds.

    I used to think the nucleotides helped stabilize the aa's. Now I think it is the other way around
    and the aa's were key in stabilizing nucleotides.

    Comment on any of this speculative idea?

    Tom

  2. << And now to the interesting part. Let's say the strand is folded with two naked ends sticking out.
    If those 2 ends are A or G, then it is likely we might have this type of coding: GA_, or GG_ Note
    the center base is the key one and the rest are the more stable G with 3 h-bonds.

    Also note that the N or _ base is wobble position. It may well have been that base farthest from the
    folded part of the strand - and the most shakey.

    Reply:

    A fellow poster has pointed out to me that base stacking would play a part in this 3 naked base
    stability. Let's say there are at least 3 bases beyond the paired regions .Then that naked base next
    to the paired body of the proto tRNA (on both the 3 and 5 ends of the strand) would extend the base
    stacking of the helix and have more base stacking stability if it was a purine. Same for the most
    important center position (on either end) and least importance for the 3rd out or wobbly positioned
    one (on either end).

    Tom

  3. << More and more I'm wondering if before the RNA world there might have been a purine world -
    A and G. >>

    TH Here's my growing list that seems to support the Purine World idea. I welcome any comment

    1. No UV dimer with purines
    2. Better stength in stacking bases with purines - specially when bases are not paired
    3. A and G most prevalent of the bases made in Miller type experiments
    4. With G there is no chirality problem though there is with A. Perhaps A sets the L-Amino
    acid spin.

    Also this part is curious; If you use G+Purine+base you get either GGN = glycine with 1 carboxyl
    group GAN = aspartic acid, or glutamic acid = 2 carboxyl groups.

    Comment?

  4. Purine World idea:

    Note this, in looking at the 2nd letter of the genetic code:

    Most A and G in 2nd "have charged, or water loving (polar) side chains and are much more chemically
    active than (C or U in 2nd position)
    - The Spark of Life.

    Comment?

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