General fitness, health and nutrition · Public discussion

Meat / good ? / bad ?

Doe · 2004-06-07T08:27:57+00:00

Since they have shown AND .. stated .. vegetables should be eaten with meat .. DUE TO .. the fact meat causes oxidation in the gut and the vegetables / antioxidants diminish this oxidation .. would this study SHOW CLEARLY .. meat is a bad .. thing .. ? http://tinyurl.com/2uh4m Am J Physiol Cell Physiol. 2004 Jun 2 [Epub ahead of print] Related Articles, Links Novel Effect of NF-{kappa}B Activation --Carbonylation and Nitration Injury to The Cytoskeleton and Disruption of Monolayer Barrier in Intestinal Epithelium. Banan A, Zhang L, Shaikh M, Fields JZ, Farhadi A, Keshavarzian A. Medicine / Gastroenterology, Molecular Physiology and Pharmacology, Rush University of Chicago, School of Medicine, Chicago, IL, USA. Using monolayers of intestinal cells, we reported that upregulation of iNOS is required for oxidative injury and that activation of NF-kappaB is key to cytoskeletal instability. In the current study we hypothesized that NF- kappaB activation is crucial to oxidant-induced iNOS upregulation and its injurious consequences, cytoskeletal oxidation & nitration and monolayer dysfunction. Wild type (WT) cells were pretreated with inhibitors of NF-kappaB +/- oxidant (H2O2). Other cells were transfected with an I- kappaBalpha mutant (an inhibitor of NF-kappaB). RESULTS: {A} Relative to WT cells exposed to vehicle, oxidant exposure caused increases in I-kappaBalpha instability; NF- kappaB subunits activation; iNOS-related activity [NO, oxidative stress, tubulin nitration]; microtubule disassembly & instability [increased monomeric & decreased polymeric tubulin]; and monolayer disruption. {B} Monolayers pretreated with NF-kappaB inhibitors (MG-132, lactacystin) were protected against oxidation, showing decreases in all measures of the NF-kappaB-->iNOS-->NO pathway. {C} Dominant mutant stabilization of I-kappaBalpha to inactivate NF-kappaB suppressed all measures of the iNOS/NO upregulation while protecting monolayers against oxidant insult. In these mutants, we found prevention of tubulin nitration & oxidation and enhancement of cytoskeletal and monolayer stability. CONCLUSIONS: [1] NF- kappaB is required for oxidant-induced iNOS upregulation and the consequent nitration and oxidation of cytoskeleton. [2] NF-kappaB activation causes cytoskeletal injury following upregulation of NO driven processes. [3] The molecular event underlying the destabilizing effects of NF- kappaB appears to be increases in carbonylation and nitrotyrosination of the subunit components of cytoskeleton. The ability to promote NO overproduction and cytoskeletal nitration/oxidation is a novel mechanism not previously attributed to NF-kappaB in cells. PMID: 15175222 [PubMed - as supplied by publisher] ------------------------------------------------------------ -------------- ------ Who loves ya. Tom Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com Man Is A Herbivore! http://pages.ivillage.com/ironjustice/manisaherbivore DEAD PEOPLE WALKING http://pages.ivillage.com/ironjustice/deadpeoplewalking

Started by Doe · 7 June 2004 · Last activity 7 June 2004 · 3 posts · 798 views

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Original section: General fitness, health and nutrition
Published: 7 June 2004
Last activity: 7 June 2004
Original author: Doe
Posts: 3
Discussion status: Public discussion
Total views: 798
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Original post
Doe
Archived post

7 June 2004, 08:27 UTC #1

Since they have shown AND .. stated .. vegetables should be
eaten with meat .. DUE TO .. the fact meat causes oxidation
in the gut and the vegetables / antioxidants diminish this
oxidation .. would this study SHOW CLEARLY .. meat is a bad
.. thing .. ?
tinyurl.com2uh4m
Am J Physiol Cell Physiol. 2004 Jun 2 [Epub ahead of print]
Related Articles, Links
Novel Effect of NF-{kappa}B Activation --Carbonylation and
Nitration Injury to The Cytoskeleton and Disruption of
Monolayer Barrier in Intestinal Epithelium.
Banan A, Zhang L, Shaikh M, Fields JZ, Farhadi A,
Keshavarzian A.
Medicine / Gastroenterology, Molecular Physiology and
Pharmacology, Rush University of Chicago, School of
Medicine, Chicago, IL, USA.
Using monolayers of intestinal cells, we reported that
upregulation of iNOS is required for oxidative injury and
that activation of NF-kappaB is key to cytoskeletal
instability. In the current study we hypothesized that NF-
kappaB activation is crucial to oxidant-induced iNOS
upregulation and its injurious consequences, cytoskeletal
oxidation & nitration and monolayer dysfunction. Wild type
(WT) cells were pretreated with inhibitors of NF-kappaB +/-
oxidant (H2O2). Other cells were transfected with an I-
kappaBalpha mutant (an inhibitor of NF-kappaB). RESULTS:
{A} Relative to WT cells exposed to vehicle, oxidant
exposure caused increases in I-kappaBalpha instability; NF-
kappaB subunits activation; iNOS-related activity [NO,
oxidative stress, tubulin nitration]; microtubule
disassembly & instability [increased monomeric & decreased
polymeric tubulin]; and monolayer disruption. {B}
Monolayers pretreated with NF-kappaB inhibitors (MG-132,
lactacystin) were protected against oxidation, showing
decreases in all measures of the NF-kappaB-->iNOS-->NO
pathway. {C} Dominant mutant stabilization of I-kappaBalpha
to inactivate NF-kappaB suppressed all measures of the
iNOS/NO upregulation while protecting monolayers against
oxidant insult. In these mutants, we found prevention of
tubulin nitration & oxidation and enhancement of
cytoskeletal and monolayer stability. CONCLUSIONS: [1] NF-
kappaB is required for oxidant-induced iNOS upregulation
and the consequent nitration and oxidation of cytoskeleton.
[2] NF-kappaB activation causes cytoskeletal injury
following upregulation of NO driven processes. [3] The
molecular event underlying the destabilizing effects of NF-
kappaB appears to be increases in carbonylation and
nitrotyrosination of the subunit components of
cytoskeleton. The ability to promote NO overproduction and
cytoskeletal nitration/oxidation is a novel mechanism not
previously attributed to NF-kappaB in cells.
PMID: 15175222 [PubMed - as supplied by publisher]
------------------------------------------------------------
--------------
------
Who loves ya. Tom Jesus Was A Vegetarian!
jesuswasavegetarian.7h.comjesuswasavegetarian.7h.com Man Is A Herbivore!
pages.ivillage.commanisaherbivore DEAD
PEOPLE WALKING
pages.ivillage.comdeadpeoplewalking

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Dec
Archived post

7 June 2004, 08:28 UTC #2

"doe" <[email hidden]> wrote in message "]news:[email hidden]...
Quoted message said:
Since they have shown AND .. stated .. vegetables should
be eaten with meat .. DUE TO .. the fact meat causes
oxidation in the gut and the vegetables / antioxidants
diminish this oxidation .. would this study SHOW CLEARLY
.. meat is a bad .. thing .. ?
tinyurl.com2uh4m
Am J Physiol Cell Physiol. 2004 Jun 2 [Epub ahead of
print] Related Articles, Links
Novel Effect of NF-{kappa}B Activation --Carbonylation and
Nitration Injury to The Cytoskeleton and Disruption of
Monolayer Barrier in Intestinal Epithelium.
Some flesh has some good stuff--long chain omega3s, and
virtually all flesh foods are good sources of B12. Most
evidence points to a largely, but not necessarily
completely, plant-based diet, say 80-95% plants with
emphasis on vegetables and fruits for optimal health.
But, there should be at least theoretical benefits to eating
exclusively plants, provided that appropriate
supplementation is maintained, since some toxics tend to
bioaccumulate with each successive trophic level in the food
chain. There is a problem, though, for people who eat only
plants: what exactly do we need to supplement with, and how
much, and are we absolutely certain that these are all that
we need if we do not eat flesh? Notice I wrote we, because I
am one of them.
-B12: latest recs are as high as 10 mcg/day.
-long chain omega3s: There is some evidence that veg*ns may
have depressed levels compared to ominvores. 650 mg/day is
enough; these do not have to be from fish, there are algae-
based sources for DHA.
-D, if sun exposure is insufficient to maintain adequate
status or intrinsic skin pigmentation is high.
other possibilities:
-taurine, arginine--these tend to be in short supply for
those who follow veg*n diets, even though the body can
synthesize them. See McCarty's recent musings in the Med
Hypothesis journal for details.

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William A. Noye
Archived post

7 June 2004, 08:28 UTC #3

Quoted message said:

Some flesh has some good stuff--long chain omega3s, and
virtually all flesh foods are good sources of B12. Most
evidence points to a largely, but not necessarily
completely, plant-based diet, say 80-95% plants with
emphasis on vegetables and fruits for optimal health.
But, there should be at least theoretical benefits to
eating exclusively plants, provided that appropriate
supplementation is maintained, since some toxics tend to
bioaccumulate with each successive trophic level in the
food chain. There is a problem, though, for people who eat
only plants: what exactly do we need to supplement with,
and how much, and are we absolutely certain that these are
all that we need if we do not eat flesh? Notice I wrote
we, because I am one of them.
-B12: latest recs are as high as 10 mcg/day.
-long chain omega3s: There is some evidence that veg*ns
may have depressed levels compared to ominvores. 650
mg/day is enough; these do not have to be from fish, there
are algae-based sources for DHA.
-D, if sun exposure is insufficient to maintain adequate
status or intrinsic skin pigmentation is high.
other possibilities:
-taurine, arginine--these tend to be in short supply for
those who follow veg*n diets, even though the body can
synthesize them. See McCarty's recent musings in the Med
Hypothesis journal for details.
l-carnitine would be another.
Ocean going fish are sources of iodine.
Plants have there own set of natural toxics. Phytoestrogens
may not be all that good for males. Perhaps there is another
reason for why primative societies the male is the hunter
beyond his male strength and his non-child bearing status.
Reason being that hunter males procreate more successfully.
Just an idea............

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