Gene Quantification Newsletter December 2003
New features on the Gene-Quantification web page:
Influence of zinc deficiency on the mRNA expression of zinc transporters in adult rats. wzw.tum.degeneOpen ↗
quantification/zinc.html
The mRNA expression of the members of the IGF-system in bovine corpus luteum during induced
luteolysis Abundance of message for insulin-like growth factors-I and -II and for receptors for
growth hormone, insulin-like growth factors-I and -II, and insulin in the intestine and liver of pre-
and full-term calves. Effects of dexamethasone and colostrum intake on the somatotropic axis in
neonatal calves. The mRNA expression of the members of the IGF-system in bovine corpus luteum during
induced luteolysis. wzw.tum.deendo.htmlOpen ↗
Expression and localisation of oestrogen and progesterone receptors in the bovine mammary gland
during development, function and involution. The gastrointestinal tract as target of steroid hormone
action: Quantification of steroid receptor mRNA expression (AR, ER-alpha, ER-beta and PR) in 10
bovine gastrointestinal tract compartments by kinetic RT-PCR. wzw.tum.degeneOpen ↗
quantification/endo.html#erab
Detection and quantification of mRNA-expression of alpha- and beta-adrenergic receptor subtypes in
the mammary gland of dairy cows. Milking characteristics and their relation to adrenergic receptor
mRNA expression and ligand binding in the mammary gland of dairy cows. wzw.tum.degeneOpen ↗
quantification/endo.html#adrenerg
New Section BioInformatics in real-time PCR wzw.tum.degeneOpen ↗
quantification/bioinformatics.shtml
Determination of real-time PCR efficiency wzw.tum.deefficiency.htmlOpen ↗
PCR Efficiency estimation via "experimental four parametric logistic model fit" (including all
fluorescence data points, Tichopad et al. 2003 - Standardized determination of real-time PCR
efficiency from a single reaction set-up.) wzw.tum.deefficiency.htmlOpen ↗
3
Erratum of Figure 2 Tichopad et al. 2003 - wzw.tum.degeneOpen ↗
quantification/efficiency.html#correction
Improving quantitative real-time RT-PCR reproducibility by boosting primer-linked amplification
efficiency wzw.tum.deefficiency.htmlOpen ↗
KOD (kinetic outlier detection) in real-time PCR (Tzachi Bar et al.,
2003)wzw.tum.deefficiency.htmlOpen ↗
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Further, a lot of NEW real-time PCR workshops wzw.tum.degeneOpen ↗
quantification/main.shtml#work
NEW PUBLICATIONS about kinetic PCR wzw.tum.dereal time.htmlOpen ↗ and wzw.tum.degeneOpen ↗
quantification/literature.html
Please send the NEWSLETTER and the home page links to further scientists and friends who are
interested in real-time PCR, new quantification strategies, relative or absolute gene quantification
using kinetic PCR.
best regards
Michael Pfaffl
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First International qPCR Symposium & Application Workshop Transcriptomics, Clinical Diagnostics &
Gene Quantification3rd - 6th March, 2004 in Freising-Weihenstephan, Germany
Up to now around 135 Abstracts submitted from 27 Nations worldwide wzw.tum.demainOpen ↗
2004.shtml#news
The Symposium and the Application Workshop presents unbiased international lectures & posters about
quantification strategies, normalisation methods & new algorithms for exact nucleic acid
quantification in the broad range of qPCR applications: Transcriptomics, Clinical Diagnostics, Food
Hygiene & GMO, Expression profiling, Microbiology, Pharmacogenomics, Forensic, Nutrigenomics,
Molecular Endocrinology, cDNA Array Verification, Theoretical qPCR, Pre-analytical Steps & RT,
Isolation of Nucleic Acids.http://www.wzw.tum.de/gene-quantification/qpcr2004/qpcr2004-workshop.html
More than 40 internationally renown speakers will be participating in a lively and exciting
programme enabling the valuable exchange of information in the qPCR field. As a special guest we can
announce Prof. Carl Wittwer from the Institute of pathology at the University of Utah School of
Medicine. He is an associate editor of Clinical Chemistry, and best known for developing techniques
in rapid-cycle PCR and as the inventor of the LightCycler. wzw.tum.demainOpen ↗
2004.shtml#speaker Parallel to the Symposium an Industrial Exhibition will take place along with the
congress, where an intensive conversation with numerous companies is possible (real-time PCR
Cyclers, Kit Producers, Nucleic Acid Purification Systems, Enzyme Producers, Plastic ware Suppliers,
etc.). 23 companies agreed to participate at the Industrial Exhibition. wzw.tum.degeneOpen ↗
quantification/qpcr2004/companies.htmlThe organisation team thank all Sponsors of the Symposium: wzw.tum.deqpcr2004Open ↗
sponsors.html
Platinum Sponsor – Roche Diagnostics Gold Sponsor – BioRad Gold Sponsor – MJ Research Silver Sponsor
– ABI Applied Biosystems Silver Sponsor – Invitrogen Silver Sponsor – Qiagen Silver Sponsor –
Stratagene
Symposium organisation & registration:
Registration Form (PDF) wzw.tum.deregistration.pdfOpen ↗
Registration Form (DOC) wzw.tum.deregistration.docOpen ↗