General fitness, health and nutrition · Public discussion

Dimers and Stop Codons

Started by Tomhendricks474 · · Last activity · 2 posts · 1,593 views

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General fitness, health and nutrition
Published
5 July 2004
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5 July 2004
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Tomhendricks474
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  1. This post continues my look at the genetic code with the
    idea that dimers may have had a major impact on its
    development (Dimer being between any two pyrimidines on the
    same strand of RNA caused by UV light damage.)

    The stop codons are UAA, UAG, UGA UGG (is tryptophan and may
    well have initially fit in here too)

    Note all the above have
    1. U+purine+purine That suggests that the anti codon on the
    tRNA had the complementary base pairing of

    A+pyrimidine+pyrimidine and those two pyrimidines would be
    a likely candidate for a dimer damage thus stopping the
    coding process.

    Therefore stop codons may stop because it sets up two
    pyrimidines in a row - and they turn into a dimer that can't
    be coded. End of genetic message.*

    2. No C That suggests why CAA, CAG, CGA, CGG, the other
    pyrimidine, +purine+purine are not stop codons.
    Deamination may well have turned C to U and kept C out of
    the code at the start - at least until this was frozen.

    ***
    And what about the start codon?

    It may also have a possible dimer clue. Start = AUG This is
    purine,pyrimidine, purine, (and again no C)
    Note: there is no chance for two pyrimidines to be adjacent
    in the codon or with the bases on either side of the
    codon. (though it is also true that GUA should work
    and it does not)

    All this is certainly speculative, yet it seems to also be
    falling into place quite nicely.

    How do others feel about dimers as being a key to the
    development of the genetic code from this and the numerous
    other posts exploring this possibility?

    *One comment was that dimers could not be replicated. Yet
    thematching strand can be, and IF the dimer was on the
    anticodon - tRNA side, any coding would probably work
    reasonably well.

  2. On Sun, 27 Jun 2004 05:00:13 +0000 (UTC),
    [email hidden] (TomHendricks474) wrote: ...
    [snip] ...

    Quoted message said:


    How do others feel about dimers as being a key to the
    development of the genetic code from this and the numerous
    other posts exploring this possibility?


    I don't like it and from the lack of response apparently
    others may not either. It may not be possible to really
    talk from out different timelines about the reasons. You
    see the development of the genetic code occurring early
    before dimer repair mechanisms exist and I see it coming
    late in the RNA world when mismatch strand repair
    mechanisms (including recognizing a dimer mismatch) would
    have been used. I'll just mention a couple things from
    earlier posts that caught my attention.
    (1) You talk of how dimers might help explain wobble in the
    3rd codon/1st anti-codon position pairing and give some
    reasons. This struck me as a very backward way to see a
    problem. By now you know that looking at random rna
    strands and how they might bind in mini-helixes you
    expect to see wobble. G=U pairings contribute as much
    energy as an A=U pairing to these short helix
    formations. In the ribosome, 100's of random tRNAs try
    to bind with a codon on an mRNA before one is
    successful. The problem that requires explanation is
    NOT why wobble is observed in one position but rather
    why there is no wobble in the other two? The current
    incomplete explanation is that this not just a dance
    between codon and tRNA but the ribosome has its h-
    bonding fingers in there also.
    (2) In another you mention I(Inosine) as a replacement for
    A as if it may have been used in the tRNAs(1st anti-
    codon position) from some early time. Inosine is
    essentially only used by eukaryotes and is most
    certainly a replacement for the G which is used in
    eubacteria. A is just not used. Likely this transition
    from G to I in eukaryotes occurred only after the
    genetic code was "frozen". I(Inosine) in a tRNA is not
    something that would be found while the genetic code
    was still forming and in its early stages (meaning not
    present in the timeline you use). William L Hunt ...
    [snip] ...

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