<<snip>> If, however, FeCl(2) with final concentration 10
microg/ml was added to the lymphocyte suspensions to
stimulate ROS production, after both durations of exposure,
the magnitude of fluorescence (ROS level during the
experiment) was significantly greater in the exposed
lymphocytes. <<snip>>
Bioelectromagnetics. 2004 Jul;25(5):324-8. Related
Articles, Links
Acute exposure to 930 MHz CW electromagnetic radiation in
vitro affects reactive oxygen species level in rat
lymphocytes treated by iron ions.
Zmyslony M, Politanski P, Rajkowska E, Szymczak W, Jajte J.
Physical Hazards Department, Nofer Institute of Occupational
Medicine, Lodz, Poland.
The aim of this study was to test the hypothesis that the
930 MHz continuous wave (CW) electromagnetic field, which is
the carrier of signals emitted by cellular phones, affects
the reactive oxygen species (ROS) level in living cells. Rat
lymphocytes were used in the experiments. A portion of the
lymphocytes was treated with iron ions to induce oxidative
processes. Exposures to electromagnetic radiation (power
density 5 W/m(2), theoretical calculated SAR = 1.5 W/kg)
were performed within a GTEM cell. Intracellular ROS were
measured by the fluorescent probe dichlorofluorescin
diacetate (DCF-DA). The results show that acute (5 and 15
min) exposure does not affect the number of produced ROS.
If, however, FeCl(2) with final concentration 10 microg/ml
was added to the lymphocyte suspensions to stimulate ROS
production, after both durations of exposure, the magnitude
of fluorescence (ROS level during the experiment) was
significantly greater in the exposed lymphocytes. The
character of the changes in the number of free radicals
observed in our experiments was qualitatively compatible
with the theoretical prediction from the model of
electromagnetic radiation effect on radical pairs.
Bioelectromagnetics
25:324-328, 2004. Copyright 2004 Wiley-Liss, Inc.
PMID: 15197754 [PubMed - in process]
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