<<snip>>
If, however, FeCl(2) with final concentration 10 microg/ml was added to the
lymphocyte suspensions to stimulate ROS production, after both durations of
exposure, the magnitude of fluorescence (ROS level during the experiment) was
significantly greater in the exposed lymphocytes.
<<snip>>
Bioelectromagnetics. 2004 Jul;25(5):324-8. Related Articles, Links
Acute exposure to 930 MHz CW electromagnetic radiation in vitro affects
reactive oxygen species level in rat lymphocytes treated by iron ions.
Zmyslony M, Politanski P, Rajkowska E, Szymczak W, Jajte J.
Physical Hazards Department, Nofer Institute of Occupational Medicine, Lodz,
Poland.
The aim of this study was to test the hypothesis that the 930 MHz continuous
wave (CW) electromagnetic field, which is the carrier of signals emitted by
cellular phones, affects the reactive oxygen species (ROS) level in living
cells. Rat lymphocytes were used in the experiments. A portion of the
lymphocytes was treated with iron ions to induce oxidative processes. Exposures
to electromagnetic radiation (power density 5 W/m(2), theoretical calculated
SAR = 1.5 W/kg) were performed within a GTEM cell. Intracellular ROS were
measured by the fluorescent probe dichlorofluorescin diacetate (DCF-DA). The
results show that acute (5 and 15 min) exposure does not affect the number of
produced ROS. If, however, FeCl(2) with final concentration 10 microg/ml was
added to the lymphocyte suspensions to stimulate ROS production, after both
durations of exposure, the magnitude of fluorescence (ROS level during the
experiment) was significantly greater in the exposed lymphocytes. The character
of the changes in the number of free radicals observed in our experiments was
qualitatively compatible with the theoretical prediction from the model of
electromagnetic radiation effect on radical pairs. Bioelectromagnetics
25:324-328, 2004. Copyright 2004 Wiley-Liss, Inc.
PMID: 15197754 [PubMed - in process]
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