Am J Vet Res. 2004 Jan; 65(1): 80-7. Related Articles, Links
Pulmonary epithelial lining fluid and plasma ascorbic acid concentrations in
horses affected by recurrent airway obstruction.
Deaton CM, Marlin DJ, Smith NC, Harris PA, Roberts CA, Schroter RC, Kelly FJ.
Centre for Equine Studies, Animal Health Trust, Lanwades Park, Kentford,
Suffolk, CB8 7UU, UK.
OBJECTIVE: To determine the pulmonary epithelial lining fluid (ELF)
concentrations and degree of oxidation of ascorbic acid in horses affected by
recurrent airway obstruction (RAO) in the presence and absence of neutrophilic
airway inflammation. ANIMALS: 6 RAO-affected horses and 8 healthy control
horses. PROCEDURE: Nonenzymatic antioxidant concentrations were determined in
RBC, plasma, and ELF samples of control horses and RAO-affected horses in the
presence and absence of airway inflammation. RESULTS: ELF ascorbic acid
concentration was decreased in RAO-affected horses with airway inflammation
(median, 0.06 mmol/L; 25th and 75th percentiles, 0.0 and 0.4 mmol/L), compared
with RAO-affected horses without airway inflammation (1.0 mmol/L; 0.7 and 1.5
mmol/L) and control horses (2.2 mmol/L; 1.4 and 2.2 mmol/L). Epithelial lining
fluid ascorbic acid remained significantly lower in RAO-affected horses without
airway inflammation than in control horses. Moreover, the ELF ascorbic acid
redox ratio (ie, ratio of the concentrations of dehydroascorbate to total
ascorbic acid) was higher in RAO-affected horses with airway inflammation
(median, 0.85; 25th and 75th percentiles, 0.25 and 1.00), compared with
RAO-affected horses without airway inflammation (0.04; 0.02 and 0.22). The
number of neutrophils in bronchoalveolar lavage fluid was inversely related to
the ELF ascorbic acid concentration (r = -0.81) and positively correlated with
the ascorbic acid redox ratio (r = 0.65). CONCLUSIONS AND CLINICAL RELEVANCE:
Neutrophilic inflammation in horses affected by RAO is associated with a
reduction in the ELF ascorbic acid pool. Nutritional supplementation with
ascorbic acid derivatives in horses affected by RAO is an area for further
investigation.
PMID: 14719707 [PubMed - in process]
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